Overview
Type: Synthetic peptide
Form: Lyophilized powder
CXCR4 (extracellular) Blocking Peptide (#BLP-CR014) is the original antigen used for immunization during Anti-CXCR4 (extracellular) Antibody (#ACR-014) generation. The blocking peptide binds and ‘blocks’ Anti-CXCR4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-CXCR4 (extracellular) Antibody (#ACR-014), (1:200).
3,4. Anti-CXCR4 (extracellular) Antibody, preincubated with CXCR4 (extracellular) Blocking Peptide (#BLP-CR014).
Expression of CXCR4 in rat cerebellumImmunohistochemical staining of rat cerebellum frozen section using Anti-CXCR4 (extracellular) Antibody (#ACR-014), (1:100). CXCR4 is expressed in Purkinje cell bodies and axonal prolongations (arrows). Hoechst 33342 is used as the counterstain.
Expression of CXCR4 in rat brainImmunohistochemical staining of rat temporal cortex using Anti-CXCR4 (extracellular) Antibody (#ACR-014), (1:400) followed by donkey anti-rabbit-biotin and streptavidin labeled with Cy3. A. CXCR4 staining (red) appears in neuronal soma and processes (arrows). DAPI is used as the counterstain (blue).
Properties
Sequence
- (C)EGISIYTSDNYTEE, corresponding to amino acid residues 2-15 of human CXCR4 (Accession P61073).
Accession (Uniprot) Number P61073
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
