CXCR4 (extracellular) Blocking Peptide (#BLP-CR014) is the original antigen used for immunization during Anti-CXCR4 (extracellular) Antibody (#ACR-014) generation. The blocking peptide binds and ‘blocks’ Anti-CXCR4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
- Expression of CXCR4 in rat cerebellumImmunohistochemical staining of rat cerebellum frozen section using Anti-CXCR4 (extracellular) Antibody (#ACR-014), (1:100). CXCR4 is expressed in Purkinje cell bodies and axonal prolongations (arrows). Hoechst 33342 is used as the counterstain.
- Expression of CXCR4 in rat brainImmunohistochemical staining of rat temporal cortex using Anti-CXCR4 (extracellular) Antibody (#ACR-014), (1:400) followed by donkey anti-rabbit-biotin and streptavidin labeled with Cy3. A. CXCR4 staining (red) appears in neuronal soma and processes (arrows). DAPI is used as the counterstain (blue).
- (C)EGISIYTSDNYTEE, corresponding to amino acid residues 2-15 of human CXCR4 (Accession P61073).