DARPP32 Blocking Peptide (#BLP-DR006) is the original antigen used for immunization during Anti-DARPP32 Antibody (#ADR-006) generation. The blocking peptide binds and ‘blocks’ Anti-DARPP32 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
- Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 3) and human HT-29 colorectal adenocarcinoma cell line lysate (lanes 2 and 4):
- Expression of DARPP32 in rat striatumImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-DARPP32 Antibody (#ADR-006), (1:200), followed by goat anti-rabbit-AlexaFluor-488. DARPP32 immunoreactivity (green) appears in striatal cells (arrows). ST=striatum, CC=corpus callosum. Cell nuclei are stained with DAPI (blue).
- (C)EGPWERPPPLDESER, corresponding to amino acid residues 160 - 174 of human DARPP32 (Accession Q9UD71).