Overview
Type: Synthetic peptide
Form: Lyophilized powder
DARPP32 Blocking Peptide (#BLP-DR006) is the original antigen used for immunization during Anti-DARPP32 Antibody (#ADR-006) generation. The blocking peptide binds and ‘blocks’ Anti-DARPP32 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1, 2. Anti-DARPP32 Antibody (#ADR-006), (1:200).
3, 4. Anti-DARPP32 Antibody, preincubated with DARPP32 Blocking Peptide (#BLP-DR006).
Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 3) and human HT-29 colorectal adenocarcinoma cell line lysate (lanes 2 and 4):1, 2. Anti-DARPP32 Antibody (#ADR-006), (1:200).
3, 4. Anti-DARPP32 Antibody, preincubated with DARPP32 Blocking Peptide (#BLP-DR006).
Expression of DARPP32 in rat striatumImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-DARPP32 Antibody (#ADR-006), (1:200), followed by goat anti-rabbit-AlexaFluor-488. DARPP32 immunoreactivity (green) appears in striatal cells (arrows). ST=striatum, CC=corpus callosum. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)EGPWERPPPLDESER, corresponding to amino acid residues 160 - 174 of human DARPP32 (Accession Q9UD71).
Accession (Uniprot) Number Q9UD71
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
