This product is freeze dried. All water molecules have been removed.
Every lot is tried & tested in a relevant biological assay.
- Banke, T.G. (2011) Brain Res. 1381, 21.
- Alomone Labs DMA inhibits Allyl isothiocyanate induced calcium signal in TRPA1 expressing cells.Dose-response curve for the inhibition of human TRPA1 expressing HEK-293 cells loaded with Calcium 5 dye for changes in intracellular Ca2+ measurements. Allyl isothiocyanate (100 µM) induced calcium signal was inhibited by 50, 100 and 200µM of DMA (#D-165), with estimated IC50 of about 60 µM.
DMA is an amiloride derivative that selectively inhibits Na+/H+ exchangers in cellular membranes, resulting in retention of H+ ions and decrease of intracellular pH. Amiloride derivatives are commonly used for their diuretic and antihypertensive properties.
Several studies have reported that DMA has positive effects in pancreatic β-cells on insulin release as a result of mis-regulation of intracellular pH and enhanced insulin secretion. Thus, amiloride derivatives show promising aspect as potential therapeutic agents for type 2 diabetes. In addition, positive effects also were shown on other cell functions such as: cell membrane electrical activity, increase of intracellular Ca2+ and retention of K+1. DMA also enables glucose to induce time-dependent potentiation and enables it to occur in situations where it is normally absent1,2.
Another study has suggested the possibility of DMA protection of the heart against post-resuscitation myocardial injury3.
DMA also has the ability to inhibit the Na+/Ca2+ exchanger and to increase its currents in rat cardiomyocytes. Experimental trials have showed that DMA increases calcium sensitivity in normal and hypertrophied cardiomyocytes4.
DMA also blocks TRPA1 channels5.
DMA (#D-165) is a highly pure, synthetic, and biologically active compound.