Free shipping starts now, no minimum, no coupons required!

Certificate of Analysis

EAAT2/GLT-1 (extracellular) Blocking Peptide

Overview

Cat #: BLP-GC022
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: bl

Application key:

BL- Blocking
For research purposes only, not for human use

Properties

Properties

Sequence
  • (C)KQLGPGKKNDEVS, corresponding to amino acid residues 151-163 of rat EAAT2 (Accession P31596).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.

Applications

Applications

Blocking
  • Western blot analysis of rat cerebellum lysate:
    Western blot analysis of rat cerebellum lysate:
    1. Anti-EAAT2 (GLT-1) (extracellular) Antibody (#AGC-022), (1:500).
    2. Anti-EAAT2 (GLT-1) (extracellular) Antibody, preincubated with EAAT2/GLT-1 (extracellular) Blocking Peptide (#BLP-GC022).
  • Western blot analysis of mouse brain membranes:
    Western blot analysis of mouse brain membranes:
    1. Anti-EAAT2 (GLT-1) (extracellular) Antibody (#AGC-022), (1:200).
    2. Anti-EAAT2 (GLT-1) (extracellular) Antibody, preincubated with EAAT2/GLT-1 (extracellular) Blocking Peptide (#BLP-GC022).
  • Rat brain sections (frozen) (1:200).
  • Rat brain frozen sections (1:50).
  • Expression of EAAT2 in rat hippocampus
    Expression of EAAT2 in rat hippocampus
    Immunohistochemical staining of perfusion-fixed frozen rat hippocampus sections using Anti-EAAT2 (GLT-1) (extracellular)-ATTO Fluor-633 Antibody (#AGC-022-FR), (1:50). A. EAAT2 (magenta) is detected in clusters (arrows) along the sub-granular layer of the dentate gyrus. B. DAPI counterstain is used to visualize cell nuclei (blue). C. Merge of A and B. G = granule layer.
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    1-2. Guinea Pig Anti-EAAT2 (GLT-1) (extracellular) Antibody (#AGP-113), (1:800).
    3-4. Guinea Pig Anti-EAAT2 (GLT-1) (extracellular) Antibody, preincubated with EAAT2 (GLT-1) (extracellular) Blocking Peptide (BLP-GC022).
Last update: 19/01/2022
Shipping and Ordering information