Free shipping starts now, no minimum, no coupons required!

Certificate of Analysis

ENaC Antibody Explorer Kit

A Screening Package of ENaC Antibodies Economically Priced

Cat #: AK-570
Size: 3 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-ENaC α (SCNN1A) (extracellular) Antibody Cat #: ASC-030

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)EELDRITEQTLFD, corresponding to amino acid residues 173-185 of rat ENaCα (Accession P37089). Extracellular loop.
Homology
Mouse, human - identical.

Anti-ENaC β (SCNN1B) (extracellular) Antibody Cat #: ASC-019

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IH, WB Reactivity: M, R
Immunogen
Peptide EFNYRTIEESPANNI(C), corresponding to amino acid residues 498-513 of rat ENaCβ (Accession P37090). Extracellular.
Homology
Mouse - identical; human - 14/15 amino acid residues identical.

Anti-ENaC γ (SCNN1G) (extracellular) Antibody Cat #: ASC-011

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IH, WB Reactivity: R
Immunogen
Peptide (C)YGVKESRKRREAGS, corresponding to amino acid residues 129-142 of rat ENaCγ (Accession P37091). Extracellular.
Homology
Human - 13/17 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
Shipping and Ordering information