Overview
Type: Synthetic peptide
Form: Lyophilized powder
Endothelin Receptor A Blocking Peptide (#BLP-ER001) is the original antigen used for immunization during Anti-Endothelin Receptor A Antibody (#AER-001) generation. The blocking peptide binds and ‘blocks’ Anti-Endothelin Receptor A primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-Endothelin Receptor A Antibody (#AER-001), (1:200).
2. Anti-Endothelin Receptor A Antibody, preincubated with Endothelin Receptor A Blocking Peptide (#BLP-ER001).
Expression of ET-A in rat lungImmunohistochemical staining of paraffin embedded section of rat lung using Anti-Endothelin Receptor A Antibody (#AER-001), (1:100). ET-A is expressed both in respiratory epithelium (black arrows) and in respiratory smooth muscle (red arrows). Hematoxilin is used as the counterstain.
Expression of ET-A in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-Endothelin Receptor A Antibody (#AER-001) (A), and Anti-Endothelin Receptor A Antibody preincubated with the control peptide antigen (B). Cresyl violet is used as the counterstain.- Mouse retinas (Bramall, A.N. et al. (2013) PLoS ONE 8, e58023.)
Properties
Sequence
- (C)NHNTERSSHKDSMN, corresponding to amino acid residues 413-426 of rat ET-A (Accession P26684).
Accession (Uniprot) Number P26684
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
