Certificate of Analysis

Endothelin Receptor Basic Research Pack

All You Need for Endothelin Receptors Research
Cat #: ESB-940 12 Vials

Antibodies

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-Endothelin Receptor A Antibody Cat #: AER-001

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)NHNTERSSHKDSMN, corresponding to amino acid residues 413-426 of rat ET-A (Accession P26684). Intracellular, C-terminus.
Homology
Human, swine, bovine - 13/14 amino acid residues identical; chicken - 12/14 amino acid residues identical.

Anti-Endothelin Receptor B Antibody Cat #: AER-002

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, WB Reactivity: H, M, R
Immunogen
Peptide CEMLRKKSGMQIALND, corresponding to amino acid residues 298-314 of rat ET-B (Accession P21451). 3rd intracellular loop.
Homology
All known vertebrate sequences - identical.

Pharmacological Reagents

BQ-3020 Cat #: SPE-200

Size: 10 mg MW: 2006 Da. Purity: ≥98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
1 pM -100 nM.
Activity
BQ-3020 is a selective and potent ET-B receptor agonist1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Soluble in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Sarafotoxin S6b Cat #: SPE-160

Size: 1 mg MW: 2564 Da. Purity: ≥98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
1 pM - 100 nM.
Activity
Sarafotoxin S6b is an endothelin receptor agonist1,2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Soluble in water. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

A-192621 Cat #: A-360

Size: 10 mg Source: Synthetic MW: 558.68 Purity: >97% (HPLC)
Effective concentration
0.1-10 nM.
Activity
A-192621 is a selective antagonist of ET-B endothelin receptors, inhibiting ET-1 binding to ETA and ET-3 binding to ETB receptors with IC50 values of 4280 nM and 4.5 nM, respectively1.
In vivo, A-192621 inhibits ETB-mediated dilatory and pressor responses induced by Sarafotoxin 6c (ED50 = 30 mg/kg), and causes an elevation of arterial blood pressure and plasma ET-1 level2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored desiccated at -20°C.
Reconstitution
Soluble in DMSO or NaOH 1Eq. Centrifuge all products before use (10000 x g 5 min).
Storage after reconstitution
Up to three months at -20°C.

ACT-132577 Cat #: A-350

Size: 10 mg Source: Synthetic MW: 546.19 Purity: >98%
Effective concentration
1-100 µM.
Activity
ACT-132577, a metabolite of macitentan, is a dual antagonist of ET-A and ET-B endothelin receptors, inhibiting binding of 125I-ET-1 to recombinant ET-A and ET-B receptors with IC50 values of 3.4 nM and 987 nM, respectively1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored desiccated at -20°C.
Reconstitution
Soluble in DMSO with gentle warming. Centrifuge all products before use (10000 x g 5 min).
Storage after reconstitution
Up to three months at -20°C.

Bosentan hydrate Cat #: B-170

Size: 250 mg Source: Synthetic MW: 569.63 Purity: >99% (HPLC)
Effective concentration
0.1-100 nM.
Activity
Bosentan is a dual antagonist of the ET-A and ET-B endothelin receptors, shown to inhibit endothelin-1 radioligand binding to ET-A and ET-B with Ki of 4.7 nM and 95 nM, respectively. In vitro, it competitively inhibits aortic contractions induced by endothelin-1 and tracheal contractions induced by Sarafotoxin S6C with pA2 of 7.2 and 6.0, respectively1. Bosentan is orally bioavailable and active in vivo2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at +4°C.
Reconstitution
Soluble in DMSO. Centrifuge all products before use (10000 x g 5 min).
Storage after reconstitution
Up to three months at -20°C.

BQ-123 sodium salt Cat #: B-185

Size: 10 mg Source: Synthetic MW: 632.69 Purity: >98%
Effective concentration
10 nM – 10 µM.
Activity
BQ-123, a cyclic peptide provided as a sodium salt, is a potent and selective antagonist of ET-A endothelin receptors, exhibiting a 1000-fold selectivity for ET-A over ET-B receptors (inhibiting endothelin-1 radioligand binding to ET-A and ET-B with IC50 of 22 nM and 18 µM, respectively). In vitro, BQ-123 inhibits endothelin-1 induced contractions of porcine coronary artery with KB of 40 nM1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored desiccated at -20°C.
Reconstitution
Soluble in DMSO. Centrifuge all products before use (10000 x g 5 min).
Storage after reconstitution
Up to three months at -20°C.

BQ788 sodium salt Cat #: B-176

Size: 5 mg Source: Synthetic MW: 663.79 Purity: >95%
Effective concentration
10-100 nM.
Activity
BQ788 is a potent and highly specific ET-B receptor antagonist, exhibiting selectivity for ET-B over ET-A human receptors when inhibiting the binding of [125I]-Endothelin-1 (IC50 of 1.2 nM and 1300 nM, respectively)1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored desiccated at -20°C.
Reconstitution
Soluble in DMSO and ethanol. Centrifuge all products before use (10000 x g 5 min).
Storage after reconstitution
Up to three months at -20°C.

FR 139317 Cat #: F-180

Size: 10 mg Source: Synthetic MW: 604.75 Purity: >99%
Effective concentration
1 nM – 1 µM.
Activity
FR 139317 is a potent and selective antagonist of ET-A receptors, inhibiting Endothelin-1 radioligand binding to ET-A and ET-B with Ki of 1 nM and 7.3 µM, respectively1in vitro, FR 139317 shifted Endothelin-1-induced contractile response curve to the right with pAof 7.2 in isolated rabbit aorta2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored desiccated at -20°C.
Reconstitution
Soluble in DMSO. Centrifuge all products before use (10000 x g 5 min).
Storage after reconstitution
Up to three months at -20°C.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For more information, please refer to individual certificate of analysis for each product.