EphA3 (extracellular) Blocking Peptide (#BLP-ER013) is the original antigen used for immunization during Anti-EphA3 (extracellular) Antibody (#AER-013) generation. The blocking peptide binds and ‘blocks’ Anti-EphA3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
- Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 5), human K562 chronic myelogenous leukemia cell line lysate (lanes 2 and 6), human Malme-3M melanoma cell line lysate (lanes 3 and 7) and human HT-29 colorectal adenocarcinoma cell line lysate (lanes 4 and 8):
- Expression of EphA3 in rat and mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections with Anti-EphA3 (extracellular) Antibody (#AER-013), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. EphA3 staining (green) in rat hippocampal CA1 region is detected in the pyramidal layer (P). B. In mouse hippocampal CA1 region, EphA3 immunoreactivity (green) is observed in the pyramidal layer (P). Cell nuclei are stained with DAPI (blue).
- (C)RARGTNVTISSLK, corresponding to amino acid residues 488-500 of human EphA3 (Accession P29320).