EphA6 (extracellular) Blocking Peptide (#BLP-ER016) is the original antigen used for immunization during Anti-EphA6 (extracellular) Antibody (#AER-016) generation. The blocking peptide binds and ‘blocks’ Anti-EphA6 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
- Western blot analysis of human LNCaP prostate adenocarcinoma cell line lysate:
- Expression of EphA6 in rat parietal cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-EphA6 (extracellular) Antibody (#AER-016), (1:200), followed by goat anti-rabbit-AlexaFluor-488. EphA6 immunoreactivity (green) appears in pyramidal cells (arrows). Cell nuclei are stained with DAPI (blue).
- (C)KEHEQLTYSSTRSK, corresponding to amino acid residues 482-495 of rat EphA6 (Accession P54758).