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Evans Blue tetrasodium salt

Direct Blue 53, Azovan Blue, Evan's Blue, Diazobleu, Evablin, Dye Evans Blue, Evans Blue Dye, Geigy-blau 536, Diamine Sky Blue FF

An Antagonist of AMPA/Kainate receptors, P2X Receptors and a Potent Inhibitor of L-Glutamate Uptake

Cat #: E-110
Alternative Name Direct Blue 53, Azovan Blue, Evan's Blue, Diazobleu, Evablin, Dye Evans Blue, Evans Blue Dye, Geigy-blau 536, Diamine Sky Blue FF
Lyophilized Powder yes
  • Bioassay Tested
    • Source Synthetic
    MW: 960.8
    Purity: >90%
    Form Lyophilized powder.
    Effective concentration 50 nM - 100 µM.
    Structure
    Chemical name Tetrasodium;(6E)-4-amino-6-[[4-[4-[(2E)-2-(8-amino-1-oxo-5,7-disulfonatonaphthalen-2-ylidene)hydrazinyl]-3-methylphenyl]-2-methylphenyl]hydrazinylidene]-5-oxonaphthalene-1,3-disulfonate.
    Molecular formula C34H24N6Na4O14S4.
    CAS No.: 314-13-6
    Activity Evans Blue tetrasodium salt blocks glutamate receptors with IC50s 150 nM and 220 nM for kainate-type GluR6 and AMPA-type GluR11.
    References-Activity
    1. Price, C.J. and Raymond, L.A. (1996) Mol. Pharmacol. 50, 1665.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Water. Centrifuge all product preparations before use (10000 x g for 1 min).
    Storage of solutions Up to four weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs Evans Blue tetrasodium salt reversibly inhibits AMPA receptors heterologously expressed in Xenopus oocytes.
      Alomone Labs Evans Blue tetrasodium salt reversibly inhibits AMPA receptors heterologously expressed in Xenopus oocytes.
      Inhibitory effect of 20 µM Evans Blue tetrasodium salt (#E-110) on GluR1 currents, elicited at -80 mV holding potential by continuous stimulation with 10 µM glutamate (indicated by uppermost horizontal bar).
    References - Scientific background
    1. Seeburg, P. (1993) Trends Neurosci. 16, 359.
    2. Lessmann, V. et al. (1992) Neurosci. Lett. 146, 13.
    3. Keller, B.U. et al. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 605.
    4. Yen, L.F. et al. (2013) PLoS One 8, e68595.
    Scientific background

    Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system and detailed knowledge of the properties of glutamate receptors is essential in understanding excitatory synaptic transmission in the brain. Glutamatergic transmission at excitatory synapses is mediated by three different classes of receptor-gated ion channels: AMPA receptors (α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid), Kainate receptors and NMDA receptors (N-methyl-D-aspartate)1.

    The biphenyl derivative of 1,3-naphthalene disulfonic acid, known as Evans Blue tetrasodium salt, was found to reduce desensitization and partially block the peak amplitudes of non-NMDA-receptor mediated currents in rat thalamic neurons2, and to block kainate evoked currents of heterologously expressed recombinant AMPA receptors containing either subunit GluR1 or GluR2 (IC50 values are 220 µM)3. Evans Blue has been an important tool in many physiological and clinical investigations because of its high affinity for plasma albumin4.

    Target AMPA, Kainate, P2X receptors
    Lyophilized Powder
    Last update: 02/03/2021

    Evans Blue tetrasodium salt (#E-110) is a highly pure, synthetic, and biologically active compound.

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    For research purposes only, not for human use

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