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Certificate of Analysis

Extracellular-Targeted P2X Receptor Antibody Explorer Kit

A Screening Package of Extracellular-Targeted P2X Receptor Antibodies Economically Priced

Cat #: AK-480
Size: 7 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-P2X1 Receptor (extracellular) Antibody Cat #: APR-022

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide CRPIYEFHGLYEEK, corresponding to amino acid residues 270-283 of human P2X1 receptor (Accession P51575). Extracellular loop.
Homology
Rat, mouse - 11/14 amino acid residues identical.

Anti-P2X2 Receptor (extracellular) Antibody Cat #: APR-025

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)KSDYLKHSTFDQDS, corresponding to amino acid residues 207-220 of rat P2X2 with replacement of cysteine 214 (C214) with serine (*S) (Accession P49653). Extracellular.
Homology
Rat, mouse - 13/14 amino acid residues identical.
Will not recognize human P2X2.

Anti-P2X3 Receptor (extracellular) Antibody Cat #: APR-026

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)KGFGRYANRVMDVSD, corresponding to amino acid residues 65-79 of rat P2X3 receptor (Accession P49654). Extracellular.
Homology
Mouse – identical; human - 13/15 amino acid residues identical.

Anti-P2X4 Receptor (extracellular) Antibody Cat #: APR-024

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)RDLAGKEQRTLTK, corresponding to amino acid residues 301-313 of rat P2X4 receptor (Accession P51577). Extracellular.
Homology
Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical.

Anti-P2X5 Receptor (extracellular) Antibody Cat #: APR-027

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)HRSHTRLAEMKYE, corresponding to amino acid residues 299-311 of rat P2X5 receptor (Accession P51578).
Homology
Mouse –12/13 amino acid residues; human – 10/13 amino acid residues.

Anti-P2X6 Receptor (extracellular) Antibody Cat #: APR-028

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide CPEGEMGTYSHGIK, corresponding to amino acid residues 136-149 of rat P2X6 receptor (Accession P51579). Extracellular.
Homology
Mouse - 13/14 amino acid residues identical; human - 11/14 amino acid residues identical.

Anti-P2X7 Receptor (extracellular) Antibody Cat #: APR-008

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor (Accession Q9Z1M0). Extracellular loop.
Homology
Human, rat - identical; bovine - 14/17 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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