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Certificate of Analysis

Extracellular-Targeted TRPV Channel Antibody Explorer Kit

A Screening Package of Extracellular-Targeted TRPV Channel Antibodies Economically Priced

Cat #: AK-485
Size: 7 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-Rat TRPV1 (VR1) (extracellular) Antibody Cat #: ACC-029

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: R
Immunogen
Peptide (C)NSLPMESTPHK*SRGS, corresponding to amino acid residues 605-619 of rat TRPV1 with replacement of cysteine 616 (C616) with serine (*S) (Accession O35433). 3rd extracellular loop.
Homology
Specific to rat TRPV1.

Anti-TRPV2 (VRL1) (extracellular) Antibody Cat #: ACC-039

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)HQPSLDQPAIPSSKAT, corresponding to amino acid residues 413-428 of rat TRPV2 (Accession Q9WUD2). 1st extracellular loop.
Homology
Mouse - 15/16 amino acid residues identical.

Anti-Human TRPV2 (VRL1) (extracellular) Antibody Cat #: ACC-139

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IFC, LCI, WB Reactivity: H
Immunogen
Peptide (C)KQAAPHLKAEVGNS, corresponding to amino acid residues 417-430 of human TRPV2 (Accession Q9Y5S1). 1st extracellular loop.
Homology
Human only.

Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody Cat #: AGP-033

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)HQPSLDQPAIPSSKAT, corresponding to amino acid residues 413-428 of rat TRPV2 (Accession Q9WUD2). 1st extracellular loop.
Homology
Mouse - 15/16.
Human - will not recognize the human epitope.

Anti-TRPV3 (extracellular) Antibody Cat #: ACC-033

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)REEEAIPHPLALTHK, corresponding to amino acid residues 464-478 of human TRPV3 (Accession Q8NET8). 1st extracellular loop.
Homology
Rat - 13/15 amino acid residues identical; mouse - 12/15 amino acid residues identical.

Anti-TRPV4 (extracellular) Antibody Cat #: ACC-124

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: H, R
Immunogen
Peptide (C)EDQSN(S)TVPSYPA(S)RD, corresponding to amino acid residues 647-662 of rat TRPV4 (Accession Q9ERZ8). 3rd extracellular loop.
Homology
Rat - 14/16 amino acid residues identical; mouse - 13/16 amino acid residues identical; human - 12/16 amino acid residues identical.

Anti-Human TRPV6 (extracellular) Antibody Cat #: ACC-028

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, LCI, WB Reactivity: H
Immunogen
Peptide (C)RTNNRTSPRDN, corresponding to amino acid residues 395-405 of human TRPV6 (Accession Q9H1D0). 1st extracellular loop.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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