Overview
Type: Synthetic peptide
Form: Lyophilized powder
GABA Transporter 1/GAT-1 (extracellular) Blocking Peptide (#BLP-GT001) is the original antigen used for immunization during Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody (#AGT-001) generation. The blocking peptide binds and ‘blocks’ Anti-GABA Transporter 1/GAT-1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody (#AGT-001), (1:200).
3,4. Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody, preincubated with GABA Transporter 1/GAT-1 (extracellular) Blocking Peptide (#BLP-GT001).
Expression of GABATR in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody (#AGT-001), (1:200). A. GAT-1 staining is shown in green. B. Neurons expressing gamma amino butyric acid (GABA) were labeled with mouse Anti-parvalbumin (red). C. Merge of the two images demonstrates partial colocalization (arrows). GAT-1 appears both in the soma of Purkinje cells (vertical arrows) and in the pinceau (horizontal arrows).
Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Guinea Pig Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody (#AGT-001-GP), (1:200).
3-4. Guinea Pig Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody, preincubated with GABA Transporter 1 (GAT-1) (extracellular) Blocking Peptide (#BLP-GT001).
Expression of GABA Transporter 1 in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Guinea Pig Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody (#AGT-001-GP), (1:300), followed by goat anti-guinea pig-AlexaFluor-594. A. GAT-1 immunoreactivity (red) appears in pinceau formations (arrows) around Purkinje cells (P). B. Pre-incubation of the antibody with GABA Transporter 1 (GAT-1) (extracellular) Blocking Peptide (#BLP-GT001), suppressed staining. Cell nuclei are stained with DAPI (blue). M = molecular layer, P = Purkinje cell layer.
Expression of GABA Transporter 1 in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody (#AGT-001-GP), (1:300), followed by goat anti-guinea pig-AlexaFluor-594. A. GAT-1 immunoreactivity (red) appears in pinceau formations (arrows) around Purkinje cells (P). B. Pre-incubation of the antibody with GABA Transporter 1 (GAT-1) (extracellular) Blocking Peptide (#BLP-GT001), suppressed staining. Cell nuclei are stained with DAPI (blue). M = molecular layer, P = Purkinje cell layer.
Properties
Sequence
- (C)ERNMHQMTDGLDK, corresponding to amino acids residues 194-206 of rat GAT-1 (Accession P23978).
Accession (Uniprot) Number P23978
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
