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Certificate of Analysis

GABA Transporter Antibody Explorer Kit

A Screening Package of GABA Transporter Antibodies Economically Priced

Cat #: AK-435
Size: 7 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-BGT-1 (SLC6A12) Antibody Cat #: AGT-004

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)GEMMDQKDKDQVKD, corresponding to amino acid residues 22-35 of rat Betaine/GABA Transporter 1 (Accession P48056). Intracellular, N-terminus.
Homology
Mouse - 13/14 amino acid residues identical.

Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody Cat #: AGT-001

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)ERNMHQMTDGLDK, corresponding to amino acids residues 194-206 of rat GAT-1 (Accession P23978). 2nd  extracellular loop.
Homology
Mouse, human - identical.

Anti-GABA Transporter 2 (GAT-2) Antibody Cat #: AGT-002

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)KVEEDGTLEREQWTNK, corresponding to amino acids residues 23-38 of rat GAT-2 (Accession P31646). Intracellular, N-terminus.
Homology
Mouse - 14/16 amino acid residues identical; human - 11/16 amino acid residues identical.

Anti-GABA Transporter 3 (GAT-3) Antibody Cat #: AGT-003

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)REARDKAVHERGH, corresponding to amino acid residues 35-47 of rat GAT-3 (Accession P31647). Intracellular, N-terminus.
Homology
Mouse – identical.

Anti-Vesicular GABA Transporter (VGAT) Antibody Cat #: AGT-005

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)GEFGGHDKPKITAWE, corresponding to amino acid residues 106-120 of rat vesicular GABA transporter (Accession O35458). Cytoplasmic, N-terminus.
Homology
Mouse, human - identical.

Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody Cat #: AGP-129

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)GEFGGHDKPKITAWE, corresponding to amino acid residues 106-120 of rat vesicular GABA transporter (Accession O35458). Cytoplasmic, N-terminus.
Homology
Mouse, human - identical.

Anti-Taurine Transporter (SLC6A6) Antibody Cat #: AGT-006

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide (C)TPREPNRWAVEREG, corresponding to amino acid residues 581-594 of rat TauT (Accession P31643). Intracellular, C-terminus.
Homology
Mouse, human – identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

Note
A guinea pig polyclonal antibody (#AGP-129) is included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
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