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Certificate of Analysis

GABA(A) α Receptor Antibody Explorer Kit

A Screening Package of GABA(A) α Receptor Antibodies Economically Priced

Cat #: AK-228
Size: 14 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-GABA(A) α1 Receptor (extracellular) Antibody Cat #: AGA-001

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide QPSQDELKDNTTVFTR(C), corresponding to amino acid residues 28-43 of rat GABRA1 (Accession P62813). Extracellular, N-terminus.
Homology
Mouse - identical; human, bovine - 16/17 amino acid residues identical; chicken - 14/17 amino acid residues identical.

GABA(A) α1 Receptor (extracellular) Blocking Peptide Cat #: BLP-GA001

Size: 40 µg

Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody Cat #: AGA-001-GP (formerly AGP-083)

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide QPSQDELKDNTTVFTR(C), corresponding to amino acid residues 28-43 of rat GABRA1 (Accession P62813). Extracellular, N-terminus.
Homology
Mouse - identical; human - 16/17 amino acid residues identical.

GABA(A) α1 Receptor (extracellular) Blocking Peptide Cat #: BLP-GA001

Size: 40 µg

Anti-GABA(A) α2 Receptor Antibody Cat #: AGA-002

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)TPEPNKKPENKPA, corresponding to amino acid residues 393-405 of rat GABRA2 (Accession P23576). 2nd intracellular loop.
Homology
Mouse, human, bovine - identical.

GABA(A) α2 Receptor Blocking Peptide Cat #: BLP-GA002

Size: 40 µg

Anti-GABA(A) α3 Receptor (extracellular) Antibody Cat #: AGA-003

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, LCI, WB Reactivity: H, M, R
Immunogen
Peptide QGESRRQEPGDFVKQ(C), corresponding to amino acid residues 29-43 of human GABRA3 (Accession P34903). Extracellular, N-terminus.
Homology
Rat, mouse - identical; bovine - 14/15 amino acid residues identical.

GABA(A) α3 Receptor (extracellular) Blocking Peptide Cat #: BLP-GA003

Size: 40 µg

Anti-GABA(A) α4 Receptor (extracellular) Antibody Cat #: AGA-008

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide NSKDEKLSPENFTR(C), corresponding to amino acid residues 37-50 of rat GABRA4 with replacement of C44 with serine (Accession P28471). Extracellular, N-terminus.
Homology
Mouse, rat - 13/14 amino acid residues identical; human - 10/14 amino acid residues identical.

GABA(A) α4 Receptor (extracellular) Blocking Peptide Cat #: BLP-GA008

Size: 40 µg

Anti-GABA(A) α5 Receptor Antibody Cat #: AGA-025

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)KKERELILNKSTN, corresponding to amino acid residues 364-376 of rat GABRA5 (Accession P19969). 2nd intracellular loop.
Homology
Mouse – identical; human – 11/13 amino acid residues identical.

GABA(A) α5 Receptor Blocking Peptide Cat #: BLP-GA025

Size: 40 µg

Anti-GABA(A) α6 Receptor (extracellular) Antibody Cat #: AGA-004

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide QLEDEGNFYSENVSRILDN(C), corresponding to amino acid residues 20-37 of rat GABRA6 (Accession P30191). Extracellullar, N-terminus.
Homology
Mouse - identical; human - 17/19 amino acid residues identical.

GABA(A) α6 Receptor (extracellular) Blocking Peptide Cat #: BLP-GA004

Size: 40 µg

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

Note
A guinea pig polyclonal antibody (#AGA-001-GP) is included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
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