Overview
GABA(A) α1 Receptor (extracellular) Blocking Peptide (#BLP-GA001) is the original antigen used for immunization during Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001) generation. The blocking peptide binds and ‘blocks’ Anti-GABA(A) α1 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications
Western blot analysis of rat brain lysates:1. Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001), (1:1000).
2. Anti-GABA (A) α1 Receptor (extracellular) Antibody, preincubated with GABA(A) α1 Receptor (extracellular) Blocking Peptide (#BLP-GA001).
Expression of GABRA1 in mouse hippocampusImmunohistochemical staining of mouse hippocampus using Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001). A. Distribution of GABRA1 (red). B. Distribution of glial fibrillary acidic protein (green). C. Merge of the two images indicates that distribution of GABRA1 is restricted to neurons and their processes. DAPI is used as the counterstain (blue).
Colocalization of CaV1.2 and GABA(A) α1 Receptor in rat cerebellumImmunohistochemical staining of rat cerebellum using Guinea pig Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003-GP) and Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001). A. CaV1.2 (green) is detected in the granule layer of the cerebellum (G) and in the upper molecular layer (star). B. In the same section, GABRA1 (red) is seen in the granule layer. C. Merge of the two images reveals high degree of colocalization between CaV1.2 and GABRA1 in the granule layer.
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) membranes and human brain neuroblastoma (SH-SY5Y) (lanes 3 and 6) cell lysate:1-3. Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001-GP), (1:400).
4-6. Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody, preincubated with GABA(A) α1 Receptor (extracellular) Blocking Peptide (#BLP-GA001).Following a broad screen of secondary antibodies, the following were used for this application:
Western blot analysis:
#106-035-006 (Jackson ImmunoResearch)
#A7289 (Sigma)
Multiplex staining of KCC2 and GABA(A) α1 Receptor in rat cerebellumImmunohistochemical staining of perfusion-fixed frozen rat brain sections using rabbit Anti-KCC2 (SLC12A5) Antibody (#AKT-005), (1:400) and Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001-GP), (1:400). A. KCC2 immunoreactivity (green) is detected in both the granule layer (GL) and the molecular layer (MOL). B. GABA(A) α1 receptor staining (red) is observed mainly in the granule layer (GL). C. Merge of the two images demonstrates colocalization in the granule layer (arrows). Cell nuclei are stained with DAPI (blue).
Expression of GABA(A) α1 Receptor in rat hippocampusImmunohistochemical staining of rat hippocampal dentate gyrus sections using Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001-GP). A. GABRA1 staining (red) appears in neuronal soma (horizontal arrows in panel B) and in the neuronal processes (vertical arrows in panel B). B. GABRA1 staining merged with DAPI counterstain (blue).
Properties
- QPSQDELKDNTTVFTR(C), corresponding to amino acid residues 28-43 of rat GABRA1 (Accession P62813).
