Overview
Type: Synthetic peptide
Form: Lyophilized powder
GABA(A) β3 Receptor Blocking Peptide (#BLP-GA013) is the original antigen used for immunization during Anti-GABA(A) β3 Receptor Antibody (#AGA-013) generation. The blocking peptide binds and ‘blocks’ Anti-GABA(A) β3 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and LNCaP cell line lysate (lanes 3 and 6):1-3. Anti-GABA(A) β3 Receptor Antibody (#AGA-013), (1:1000)
4-6. Anti-GABA(A) β3 Receptor Antibody, preincubated with GABA(A) β3 Receptor Blocking Peptide (#BLP-GA013).
Expression of GABA(A) β3 in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GABA(A) β3 Receptor Antibody (#AGA-013), (1:200), followed by goat anti-rabbit-Alexa-488. A. GABA(A) β3 immunoreactivity (green) appears in the cerebellar granule layer (G). B. Pre-incubation of the antibody with GABA(A) β3 Receptor Blocking Peptide (#BLP-GA013), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)GRYMGDRSIPHKK, corresponding to amino acid residues 413-425 of rat GABRB3 (Accession P63079).
Accession (Uniprot) Number P63079
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
