Overview
Type: Synthetic peptide
Form: Lyophilized powder
GABA(A) ε Receptor/GABRE (extracellular) Blocking Peptide (#BLP-GA015) is the original antigen used for immunization during Anti-GABA(A) ε Receptor (GABRE) (extracellular) Antibody (#AGA-015) generation. The blocking peptide binds and ‘blocks’ Anti-GABA(A) ε Receptor/GABRE (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain lysate (lanes 2 and 6), human SHSY-5Y neuroblastoma cells lysate (lanes 3 and 7) and human U87 MG glioblastoma cells lysate (lanes 4 and 8):1-4. Anti-GABA(A) ε Receptor (GABRE) (extracellular) Antibody (#AGA-015), (1:400).
5-8. Anti-GABA(A) ε Receptor (GABRE) (extracellular) Antibody, preincubated with GABA(A) ε Receptor/GABRE (extracellular) Blocking Peptide (#BLP-GA015).
Expression of GABA(A) ε receptor in rat and mouse cerebellaImmunohistochemical staining of mouse and rat cerebellum using Anti-GABA(A) ε Receptor (GABRE) (extracellular) Antibody (#AGA-015). In both mouse (A) and rat (B) cerebellum GABA(A) ε receptor staining (purple) appears in the Purkinje cell body (P and vertical arrows) and in their dendritic tree (horizontal arrow) extending into the molecular layer (MOL). Nucler staining using DAPI as the counterstaining (blue).
Properties
Sequence
- (C)RLRYNDTFETLILH, corresponding to amino acid residues 131-144 of rat GABRE (Accession Q9ES14).
Accession (Uniprot) Number Q9ES14
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
