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Certificate of Analysis

GABA(A) Receptor Antibody Explorer Kit

A Screening Package of GABA(A) Receptor Antibodies Economically Priced

Cat #: AK-229
Size: 34 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-GABA(A) α1 Receptor (extracellular) Antibody Cat #: AGA-001

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide QPSQDELKDNTTVFTR(C), corresponding to amino acid residues 28-43 of rat GABRA1 (Accession P62813). Extracellular, N-terminus.
Homology
Mouse - identical; human, bovine - 16/17 amino acid residues identical; chicken - 14/17 amino acid residues identical.

GABA(A) α1 Receptor (extracellular) Blocking Peptide Cat #: BLP-GA001

Size: 40 µg

Anti-GABA(A) α2 Receptor Antibody Cat #: AGA-002

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)TPEPNKKPENKPA, corresponding to amino acid residues 393-405 of rat GABRA2 (Accession P23576). 2nd intracellular loop.
Homology
Mouse, human, bovine - identical.

GABA(A) α2 Receptor Blocking Peptide Cat #: BLP-GA002

Size: 40 µg

Anti-GABA(A) α3 Receptor (extracellular) Antibody Cat #: AGA-003

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, LCI, WB Reactivity: H, M, R
Immunogen
Peptide QGESRRQEPGDFVKQ(C), corresponding to amino acid residues 29-43 of human GABRA3 (Accession P34903). Extracellular, N-terminus.
Homology
Rat, mouse - identical; bovine - 14/15 amino acid residues identical.

GABA(A) α3 Receptor (extracellular) Blocking Peptide Cat #: BLP-GA003

Size: 40 µg

Anti-GABA(A) α4 Receptor (extracellular) Antibody Cat #: AGA-008

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide NSKDEKLSPENFTR(C), corresponding to amino acid residues 37-50 of rat GABRA4 with replacement of C44 with serine (Accession P28471). Extracellular, N-terminus.
Homology
Mouse, rat - 13/14 amino acid residues identical; human - 10/14 amino acid residues identical.

GABA(A) α4 Receptor (extracellular) Blocking Peptide Cat #: BLP-GA008

Size: 40 µg

Anti-GABA(A) α5 Receptor Antibody Cat #: AGA-025

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)KKERELILNKSTN, corresponding to amino acid residues 364-376 of rat GABRA5 (Accession P19969). 2nd intracellular loop.
Homology
Mouse – identical; human – 11/13 amino acid residues identical.

GABA(A) α5 Receptor Blocking Peptide Cat #: BLP-GA025

Size: 40 µg

Anti-GABA(A) α6 Receptor (extracellular) Antibody Cat #: AGA-004

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide QLEDEGNFYSENVSRILDN(C), corresponding to amino acid residues 20-37 of rat GABRA6 (Accession P30191). Extracellullar, N-terminus.
Homology
Mouse - identical; human - 17/19 amino acid residues identical.

GABA(A) α6 Receptor (extracellular) Blocking Peptide Cat #: BLP-GA004

Size: 40 µg

Anti-GABA(A) β1 Receptor Antibody Cat #: AGA-010

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide (C)DQSANEKNKLEMNK, corresponding to amino acid residues 345-358 of rat GABRB1 (Accession P15431). 2nd intracellular loop.
Homology
Human – identical; mouse – 13/14 amino acid residues identical.

GABA(A) β1 Receptor Blocking Peptide Cat #: BLP-GA010

Size: 40 µg

Anti-GABA(A) β3 Receptor Antibody Cat #: AGA-013

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)GRYMGDRSIPHKK, corresponding to amino acid residues 413-425 of rat GABRB3 (Accession P63079). 2nd intracellular loop.
Homology
Mouse – identical; human – 10/13 amino acid residues identical.

GABA(A) β3 Receptor Blocking Peptide Cat #: BLP-GA013

Size: 40 µg

Anti-GABA(A) γ1 Receptor Antibody Cat #: AGA-016

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)HAGSTLIPMNNISMPQGE, corresponding to amino acid residues 382-399 of rat GABA(A) γ1 receptor (Accession P23574). Intracellular, C-terminus.
Homology
Mouse - 16/18 amino acid residues identical; human - 14/18 amino acid residues identical.

GABA(A) γ1 Receptor Blocking Peptide Cat #: BLP-GA016

Size: 40 µg

Anti-GABA(A) γ2 Receptor (extracellular) Antibody Cat #: AGA-005

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide QKSDDDYEDYASNKT(C), corresponding to amino acid residues 39-53 of rat GABA(A) γ2 receptor (Accession P18508). Extracellular, N-terminus.
Homology
Human, mouse, bovine - identical; chick - 13/15 amino acid residues identical.

GABA(A) γ2 Receptor (extracellular) Blocking Peptide Cat #: BLP-GA005

Size: 40 µg

Anti-GABA(A) δ Receptor (extracellular) Antibody Cat #: AGA-014

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IFC, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)HHGARAMNDIGDYVGSN, corresponding to amino acid residues 19-35 of rat GABA(A) δ receptor (Accession P18506).
Homology
Mouse - identical; human - 14/17 amino acid residues identical.

GABA(A) δ Receptor (extracellular) Blocking Peptide Cat #: BLP-GA014

Size: 40 µg

Anti-GABA(A) ε Receptor (GABRE) (extracellular) Antibody Cat #: AGA-015

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)RLRYNDTFETLILH, corresponding to amino acid residues 131-144 of rat GABRE (Accession Q9ES14). Extracellular, N-terminus.
Homology
Mouse – identical; human - 10/14 amino acid residues identical.

GABA(A) ε Receptor/GABRE (extracellular) Blocking Peptide Cat #: BLP-GA015

Size: 40 µg

Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody Cat #: AGA-018

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide DYRMHEKLWVPDC, corresponding to amino acid residues 131-143 of mouse GABRQ (Accession Q9JLF1). Extracellular, N-terminus.
Homology
Rat, human - identical.

GABA(A) θ Receptor/GABRQ (extracellular) Blocking Peptide Cat #: BLP-GA018

Size: 40 µg

Anti-GABA(A) ρ1 Receptor (GABRR1) (extracellular) Antibody Cat #: AGA-006

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: R
Immunogen
Peptide (C)ESTVHWPGREVHE, corresponding to amino acid residues 23-35 of rat GABRR1 (Accession P50572). Extracellular, N-terminus.
Homology
Mouse, human - 12/13 amino acid residues identical.

GABA(A) ρ1 Receptor/GABRR1 (extracellular) Blocking Peptide Cat #: BLP-GA006

Size: 40 µg

Anti-GABA(A) ρ2 Receptor (GABRR2) (extracellular) Antibody Cat #: AGA-007

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: R
Immunogen
Peptide (C)RKRWTGHLETSKPSH, corresponding to amino acid residues 51-65 of rat GABRR2 (Accession P47742). Extracellular, N-terminus.
Homology
Mouse - 14/15 amino acid residues identical; human - 11/15 amino acid residues identical.

GABA(A) ρ2 Receptor/GABRR2 (extracellular) Blocking Peptide Cat #: BLP-GA007

Size: 40 µg

Anti-GABA(A) ρ3 Receptor (GABRR3) (extracellular) Antibody Cat #: AGA-020

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, LCI, WB Reactivity: M, R
Immunogen
Peptide CLSSPKQTRIRETR, corresponding to amino acid residues 31-44 of rat GABRR3 (Accession P50573). Extracellular, N-terminus.
Homology
Mouse – identical; human – 9/14 amino acid residues identical.

GABA(A) ρ3 Receptor/GABRR3 (extracellular) Blocking Peptide Cat #: BLP-GA020

Size: 40 µg

Anti-GABA(A) π Receptor (extracellular) Antibody Cat #: AGA-019

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IH, WB Reactivity: M, R
Immunogen
Peptide (C)DGNDVEFSWLRGND, corresponding to amino acid residues 184-197 of rat GABA(A) π Receptor (Accession O09028). Extracellular, N-terminus.
Homology
Mouse – identical; human – 13/14 amino acid residues identical.

GABA(A) π Receptor (extracellular) Blocking Peptide Cat #: BLP-GA019

Size: 40 µg

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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