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Genistein

A Tyrosine Kinase Inhibitor

Cat #: G-300
Lyophilized Powder yes
  • Bioassay Tested
  • Origin Glycine max (Soy bean).
    Source Natural
    MW: 270.2
    Purity: >99%
    Effective concentration 0.5-10 µM.
    Structure
    Chemical name 5,7-Dihydroxy-3-(4-hydroxyphenyl)-4H-1-benzopyran-4-one.
    Molecular formula C15H10O5.
    CAS No.: 446-72-0
    Activity Genistein, apart from a broad range of biological roles, is well established as a tyrosine kinase inhibitor1.
    References-Activity
    1. Akiyama, T. et al. (1987) J. Biol. Chem262, 5592.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to one week at 4°C or three months at -20°C.
    Protect from light.
    Our bioassay
    • Alomone Labs Genistein inhibits tyrosine kinase in HeLa cells.
      Alomone Labs Genistein inhibits tyrosine kinase in HeLa cells.
      Cells were grown to 70% confluence, and then starved for 18 h. The cells were preincubated for 30 min in the presence of 0.5 µM Genistein (#G-300) and then stimulated for 2 h with 20% fetal calf serum or culture medium. The cell extracts were blotted and probed with phospho-Tyr specific antibodies.
    References - Scientific background
    1. Polkowski, K. and Mazurek, A.P. (2000) Acta Pol. Pharm57, 135.
    2. Sarkar, F.H. and Li, Y. (2002) Cancer Metastasis Rev. 21, 265.
    3. Salti, G.I. (2000) Eur. J. Cancer36, 796.
    4. Spinozzi, F. et al. (1994) Leuk. Res18, 431.
    5. Myoung, H. et al. (2003) Cancer Sci94, 215.
    6. Li, Y. and Sarkar, F.H. (2002) Cancer Lett. 186, 157.
    7. Huang, R.Q. et al. (1999) Brain Res. Mol. Brain Res67, 177.
    8. Constantinou, A. and Huberman, E. (1995) Proc. Soc. Exp. Biol. Med208, 109.
    9. Khoshyomn, S. et al. (2002) J. Neurooncol. 57, 193.
    10. Tamura, S. et al. (2003) Pigment Cell Res. 16, 470.
    Scientific background An isoflavonoid derived from soy products,1 Genistein inhibits protein tyrosine kinases including the EGF receptor kinase (IC50 = 2.6 µM). The inhibition is competitive with respect to ATP and non-competitive with respect to the phosphate acceptor.1,2 It has a trivial effect on the activity of PKA and PKC (IC50 > 350 µM).1,2 In addition, Genistein inhibits Topoisomerase II activity,3 causes cell cycle arrest and apoptosis in Jurkat T-leukemia cells.4 It down regulates the transcription of genes involved in angiogenesis,5,6 and inhibits the action of GABA on recombinant GABA-A receptors.7 Genistein also inhibits tumor cell proliferation and induces tumor cell differentiation.8-10
    Last update: 11/04/2021

    Genistein (#G-300) is a highly pure, natural, and biologically active compound.

    For research purposes only, not for human use

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