Overview
Type: Synthetic peptide
Form: Lyophilized powder
GFR α 4 (extracellular) Blocking Peptide (#BLP-NT024) is the original antigen used for immunization during Anti-GFR alpha 4 (extracellular) Antibody (#ANT-024) generation. The blocking peptide binds and ‘blocks’ Anti-GFR α 4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-GFR alpha 4 (extracellular) Antibody (#ANT-024), (1:800).
3,4. Anti-GFR alpha 4 (extracellular) Antibody, preincubated with GFR α 4 (extracellular) Blocking Peptide (#BLP-NT024).
Expression of GFRA4 in rat red nucleusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GFR alpha 4 (extracellular) Antibody (#ANT-024), (1:100). Staining reveals expression of GFRA4 (red) in cells with neuronal outline (arrows point at some examples) in the red nucleus. DAPI is used as the counterstain (blue).
Properties
Sequence
- (C)GSRDG(S)PEEGGPR, corresponding to amino acid residues 160-172 of rat GFRA4 (Accession Q9EPI2).
Accession (Uniprot) Number Q9EPI2
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
