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Certificate of Analysis

Glucagon Receptor Family Antibody Explorer Kit

A Screening Package of Glucagon Receptor Family Antibodies Economically Priced

Cat #: AK-505
Size: 8 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-GIPR (extracellular) Antibody Cat #: AGR-025

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IFC, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)EGQTTGELYQRWER,corresponding to amino acid residues 25-38 of rat GIPR (Accession P43219). Extracellular, N-terminus.
Homology
Mouse - 13/14 amino acid residues identical; human - 12/14 amino acid residues identical.

GIPR (extracellular) Blocking Peptide Cat #: BLP-GR025

Size: 40 µg

Anti-Glucagon Receptor (extracellular) Antibody Cat #: AGR-024

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)KTRYSQKIGDDLS, corresponding to amino acid residues 200-212 of rat GCGR (Accession P30082). 1st  extracellular loop.
Homology
Mouse - identical; human - 12/13 amino acid residues identical.

Glucagon Receptor (extracellular) Blocking Peptide Cat #: BLP-GR024

Size: 40 µg

Anti-GLP1R (extracellular) Antibody Cat #: AGR-021

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)KYLYEDEG(S)WTRNS, corresponding to amino acid residues 288-301 of rat GLP1R (Accession P32301). 2nd extracellular loop.
Homology
Rat, mouse, human - 13/14 amino acid residues identical.

GLP1R (extracellular) Blocking Peptide Cat #: BLP-GR021

Size: 40 µg

Anti-GLP2R (extracellular) Antibody Cat #: AGR-022

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: R
Immunogen
Peptide (C)KRPDDESGWMSYLSE, corresponding to amino acid residues 241-255 of rat GLP2R (Accession Q9Z0W0). 1st extracellular loop.
Homology
Mouse, human - 13/15 amino acid residues identical.

GLP2R (extracellular) Blocking Peptide Cat #: BLP-GR022

Size: 40 µg

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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