GLUT1 (extracellular) Blocking Peptide (#BLP-GT041) is the original antigen used for immunization during Anti-GLUT1 (extracellular) Antibody (#AGT-041) generation. The blocking peptide binds and ‘blocks’ Anti-GLUT1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
- Western blot analysis of human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 1 and 5), human HepG2 hepatocellular carcinoma cell line lysate (lanes 2 and 6), human K562 chronic myeloid leukemia cell line lysate (lanes 3 and 7) and human Jurkat T-cell leukemia cell line lysate (lanes 4 and 8):
- (C)EEFYNQTWNHRYGES, corresponding to amino acid residues 41-55 of rat Glucose transporter 1 (Accession P11167).