Glycine Receptor α3 Blocking Peptide (#BLP-GR003) is the original antigen used for immunization during Anti-Glycine Receptor α3 Antibody (#AGR-003) generation. The blocking peptide binds and ‘blocks’ Anti-Glycine Receptor α3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-Glycine Receptor α3 Antibody (#AGR-003), (1:200).
3-4. Anti-Glycine Receptor α3 Antibody, preincubated with Glycine Receptor α3 Blocking Peptide (BLP-GR003).
- Rat trigeminal ganglion sections (1:100) (Bae, J.Y. et al. (2016) Brain Struct. Funct. 221, 4601.).
- (C)KNKTEAFALEKFYR, corresponding to amino acid residues 355-368 of rat GlyRα3 (Accession P24524).