Overview
Type: Synthetic peptide
Form: Lyophilized powder
Glycine Receptor α3 Blocking Peptide (#BLP-GR003) is the original antigen used for immunization during Anti-Glycine Receptor α3 Antibody (#AGR-003) generation. The blocking peptide binds and ‘blocks’ Anti-Glycine Receptor α3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-Glycine Receptor α3 Antibody (#AGR-003), (1:200).
3-4. Anti-Glycine Receptor α3 Antibody, preincubated with Glycine Receptor α3 Blocking Peptide (BLP-GR003).- Rat trigeminal ganglion sections (1:100) (Bae, J.Y. et al. (2016) Brain Struct. Funct. 221, 4601.).
Expression of Glycine Receptor alpha 3 in rat dorsal raphe nucleus (DRN).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Glycine Receptor α3 Antibody (#AGR-003), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. GlyRα3 immunoreactivity (green) appears in DRN neurons (arrows). B. Pre-incubation of the antibody with Glycine Receptor α3 Blocking Peptide (BLP-GR003), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of Glycine Receptor alpha 3 in rat substantia nigra pars compacta (SNC).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Glycine Receptor α3 Antibody (#AGR-003), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. GlyRα3 immunoreactivity (green) appears in outlines of neurons (arrows). B. Pre-incubation of the antibody with Glycine Receptor α3 Blocking Peptide (BLP-GR003), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KNKTEAFALEKFYR, corresponding to amino acid residues 355-368 of rat GlyRα3 (Accession P24524).
Accession (Uniprot) Number P24524
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
