GlyT1/SLC6A9 (extracellular) Blocking Peptide (#BLP-GT011) is the original antigen used for immunization during Anti-GlyT1 (SLC6A9) (extracellular) Antibody (#AGT-011) generation. The blocking peptide binds and ‘blocks’ Anti-GlyT1/SLC6A9 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human U87-MG glioma cell (lanes 3 and 6) lysates:
- (C)RLYVLKLSDDIGD, corresponding to amino acid residues 202-214 of rat GlyT1 (Accession P28572).