GlyT2/SLC6A5 (extracellular) Blocking Peptide (#BLP-GT012) is the original antigen used for immunization during Anti-GlyT2 (SLC6A5) (extracellular) Antibody (#AGT-012) generation. The blocking peptide binds and ‘blocks’ Anti-GlyT2/SLC6A5 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and rat cerebellum (lanes 3 and 6) lysates:
- Expression of glycine transporter 2 in mouse brain stemImmunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using Anti-GlyT2 (SLC6A5) (extracellular) Antibody (#AGT-012), (1:200). A. GlyT2 (red) is expressed in neurons (arrow). B. Dapi staining of cell nuclei (blue) is used as a general cellular marker.
- CVIGDHPKIQIKNS, corresponding to amino acid residues 333-346 of rat GlyT2 (Accession P58295).