Free shipping starts now, no minimum, no coupons required!

Certificate of Analysis

GPCR Pain-Related Antibody Explorer Kit

A Screening Package of GPCR Pain-Related Antibodies Economically Priced

Cat #: AK-545
Size: 8 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-Cannabinoid Receptor 1 (extracellular) Antibody Cat #: ACR-001

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide NKSLSSFKENEENIQC, corresponding to amino acid residues 84-99 of rat CB1 receptor (Accession P20272). Extracellular, N-terminus.
Homology
Human - identical; mouse - 15/16 amino acid residues identical.

Anti-Cannabinoid Receptor 2 Antibody Cat #: ACR-002

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IH, WB Reactivity: M, R
Immunogen
Peptide (C)DRQVPGIARMRLDVR, corresponding to amino acid residues 228-242 of rat CB2 receptor (Accession Q9QZN9). 3rd intracellular loop.
Homology
Mouse - identical.

Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody Cat #: ACR-003

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, LCI, WB Reactivity: H
Immunogen
Peptide (C)NGSKDGLDSNPMKD, corresponding to amino acid residues 11-24 of human CB2 receptor (Accession P34972). Extracellular, N-terminus.
Homology
Canis, bovine - 10/14 amino acid residues identical.

Anti-GPR65 (TDAG8) (extracellular) Antibody Cat #: AGR-043

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)DYTWNKDNWTFSP, corresponding to amino acid residues 72 - 84 of human GPR65 (Accession Q8IYL9). 1st extracellular loop.
Homology
Mouse – 12/13 amino acid residues identical; rat - 11/13 amino acid residues identical.

Anti-δ-Opioid Receptor (OPRD1) (extracellular) Antibody Cat #: AOR-014

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)ELVPSARAELQSSPLVN corresponding to amino acid residues 2-18 of mouse δ-Opioid receptor (Accession P32300). Extracellular, N-terminus.
Homology
Rat - 13/17 amino acid residues identical.

Anti-κ-Opioid Receptor (OPRK1) (extracellular) Antibody Cat #: AOR-012

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)NGSVGSEDQQLEPAHIS, corresponding to amino acid residues 39-55 of rat κ-Opioid receptor (Accession P34975). Extracellular, N-terminus.
Homology
Mouse -16/17 amino acid residues identical; human - 15/17 amino acid residues identical.

Anti-µ-Opioid Receptor (OPRM1) (extracellular) Antibody Cat #: AOR-011

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide CSPAPGSWLNLSHVDGN, corresponding to amino acid residues 22-38 of the rat µ-Opioid receptor (Accession P33535). Extracellular, N-terminus.
Homology
Mouse - identical; human - 15/17 amino acid residues identical.

Anti-Nociceptin Receptor (OPRL1) Antibody Cat #: AOR-015

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide (C)HREMQVSDRVRSIAKD, corresponding to amino acid residues 337-352 of rat OPRL1 (Accession P35370). Intracellular, C-terminus.
Homology
Mouse - identical; human - 13/16 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
Shipping and Ordering information