GPER1/GPR30 (extracellular) Blocking Peptide (#BLP-ER049) is the original antigen used for immunization during Anti-GPER1/GPR30 (extracellular) Antibody (#AER-049) generation. The blocking peptide binds and ‘blocks’ Anti-GPER1/GPR30 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
- Western blot analysis of human MCF-7 breast adenocarcinoma (lanes 1 and 4), human THP-1 monocytic leukemia (lanes 2 and 5) and human Jurkat T-cell leukemia (lanes 3 and 6) cell line lysates:
- CKQSFRHAYPLTGH, corresponding to amino acid residues 294 - 307 of mouse GPER1 (Accession Q8BMP4).