GPR139 Blocking Peptide (#BLP-GR069) is the original antigen used for immunization during Anti-GPR139 Antibody (#AGR-069) generation. The blocking peptide binds and ‘blocks’ Anti-GPR139 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):1-2. Anti-GPR139 Antibody (#AGR-069), (1:800).
3-4. Anti-GPR139 Antibody, preincubated with GPR139 Blocking Peptide (BLP-GR069).
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-GPR139 Antibody (#AGR-069), (1:200).
2. Anti-GPR139 Antibody, preincubated with GPR139 Blocking Peptide (BLP-GR069).
- Expression of GPR139 in rat ventromedial hypothalamus (VMH).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR139 Antibody (#AGR-069), (1:600), followed by goat anti-rabbit-AlexaFluor-488. A. GPR139 immunoreactivity (green) appears in tanycyte-like processes (arrows). B. Pre-incubation of the antibody with GPR139 Blocking Peptide (BLP-GR069), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = third ventricle.
- Peptide (C)RKSNFRLRGYSTGK, corresponding to amino acid residues 204 - 217 of mouse GPR139 (Accession Q80UC8).