GPR171 (extracellular) Blocking Peptide (#BLP-GR054) is the original antigen used for immunization during Anti-GPR171 (extracellular) Antibody (#AGR-054) generation. The blocking peptide binds and ‘blocks’ Anti-GPR171 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
- Western blot analysis of human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):
- Peptide (C)KDIKEKSNVG(S)MEFK, corresponding to amino acid residues 155 - 169 of mouse GPR171 (Accession Q8BG55).