Overview
Type: Synthetic peptide
Form: Lyophilized powder
GPR39 (extracellular) Blocking Peptide (#BLP-GR045) is the original antigen used for immunization during Anti-GPR39 (extracellular) Antibody (#AGR-045) generation. The blocking peptide binds and ‘blocks’ Anti-GPR39 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse kidney (lanes 1 and 3) and brain (lanes 2 and 4) lysates:1,2 Anti-GPR39 (extracellular) Antibody (#AGR-045), (1:200).
3,4. Anti-GPR39 (extracellular) Antibody, preincubated with GPR39 (extracellular) Blocking Peptide (#BLP-GR045).
Western blot analysis of rat colon (lanes 1 and 3) and pancreas (lanes 2 and 4) lysates:1,2 Anti-GPR39 (extracellular) Antibody (#AGR-045), (1:200).
3,4. Anti-GPR39 (extracellular) Antibody, preincubated with GPR39 (extracellular) Blocking Peptide (#BLP-GR045).
Western blot analysis of human HT-29 colorectal adenocarcinoma (lanes 1 and 3) and human MEG-01 megakaryoblastic leukemia (lanes 2 and 4) cell line lysates:1,2 Anti-GPR39 (extracellular) Antibody (#AGR-045), (1:200).
3,4. Anti-GPR39 (extracellular) Antibody, preincubated with GPR39 (extracellular) Blocking Peptide (#BLP-GR045).
Properties
Sequence
- (C)KPKHDWTRTYFR, corresponding to amino acid residues 309-320 of mouse GPR39 (Accession Q5U431).
Accession (Uniprot) Number Q5U431
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
