GPR39 (extracellular) Blocking Peptide (#BLP-GR045) is the original antigen used for immunization during Anti-GPR39 (extracellular) Antibody (#AGR-045) generation. The blocking peptide binds and ‘blocks’ Anti-GPR39 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse kidney (lanes 1 and 3) and brain (lanes 2 and 4) lysates:
- Western blot analysis of rat colon (lanes 1 and 3) and pancreas (lanes 2 and 4) lysates:
- Western blot analysis of human HT-29 colorectal adenocarcinoma (lanes 1 and 3) and human MEG-01 megakaryoblastic leukemia (lanes 2 and 4) cell line lysates:
- (C)KPKHDWTRTYFR, corresponding to amino acid residues 309-320 of mouse GPR39 (Accession Q5U431).