GPR4 (extracellular) Blocking Peptide (#BLP-GR041) is the original antigen used for immunization during Anti-GPR4 (extracellular) Antibody (#AGR-041) generation. The blocking peptide binds and ‘blocks’ Anti-GPR4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat dorsal root ganglion lysate:
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) kidney membranes:
- Expression of GPR4 in rat dorsal raphe nucleus (DRN).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR4 (extracellular) Antibody (#AGR-041), (1:400), followed by goat anti-rabbit-Alexa-488. A. GPR4 immunoreactivity (green) appears in cells of both medial and lateral DRN (arrows). B. Pre-incubation of the antibody with GPR4 (extracellular) Blocking Peptide (#BLP-GR041), suppressed staining. Cell nuclei are stained with DAPI (blue). LDRN = lateral DRN, MDRN = medial DRN.
- (C)RDRYNHTF(S)FEKFPME, corresponding to amino acid residues 162-177 of rat GPR4 (Accession Q4KLH9).