GPR83 (extracellular) Blocking Peptide (#BLP-GR053) is the original antigen used for immunization during Anti-GPR83 (extracellular) Antibody (#AGR-053) generation. The blocking peptide binds and ‘blocks’ Anti-GPR83 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
- Western blot analysis of human Jurkat T-cell leukemia cell line lysate:
- Expression of GPR83 in mouse hippocampus and cortexImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR83 (extracellular) Antibody (#AGR-053), (1:3000), followed by goat anti-rabbit-AlexaFluor-488. A. GPR83 staining (green) in hippocampal dentate gyrus is detected in hilar interneurons (vertical arrows) and around cells of the granule layer (horizontal arrows). H = hilus of dentate gyrus. G = granule layer. B. GPR83 immunoreactivity (green) in parietal cortex is observed in neurons of the pyramidal layer (vertical arrows) and in their apical dendrites (horizontal arrows). Cell nuclei are stained with DAPI (blue).
- (C)RRYGAESQNPTVK, corresponding to amino acid residues 59 - 71 of mouse GPR83 (Accession P30731).