GPR91/SUCNR1 (extracellular) Blocking Peptide (#BLP-SR090) is the original antigen used for immunization during Anti-GPR91 (SUCNR1) (extracellular) Antibody (#ASR-090) generation. The blocking peptide binds and ‘blocks’ Anti-GPR91/SUCNR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat kidney membrane (lanes 1 and 3), mouse kidney lysate (lanes 2 and 4), mouse TK-1 T-cell lymphoma (lanes 5 and 8), mouse J774 macrophage cell (lanes 6 and 9) and human Jurkat acute T cell leukemia (lanes 7 and 10) lysates:
- Expression of GPR91 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR91 (SUCNR1) (extracellular) Antibody (#ASR-090), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. GPR91 immunoreactivity (green) appears in neuronal profiles in the pyramidal layer (P, vertical arrow) and the stratum oriens (SO, horizontal arrow). B. Pre-incubation of the antibody with GPR91 (SUCNR1) (extracellular) Blocking Peptide (BLP-SR090), suppressed staining. Cell nuclei are stained with DAPI (blue).
- (C)RNVRIASRLDSWPQG, corresponding to amino acid residues 248-262 of rat GPR91/Succinate Receptor 1 (Accession Q6IYF9).