Overview
Type: Synthetic peptide
Form: Lyophilized powder
GSG1L Blocking Peptide (#BLP-GC048) is the original antigen used for immunization during Anti-GSG1L Antibody (#AGC-048) generation. The blocking peptide binds and ‘blocks’ Anti-GSG1L primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1, 2. Anti-GSG1L Antibody (#AGC-048), (1:200).
3, 4. Anti-GSG1L Antibody, preincubated with GSG1L Blocking Peptide (#BLP-GC048).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-GSG1L Antibody (#AGC-048), (1:200).
2. Anti-GSG1L Antibody, preincubated with GSG1L Blocking Peptide (#BLP-GC048).
Expression of GSG1L in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GSG1L Antibody (#AGC-048), (1:200), followed by goat anti-rabbit-AlexaFluor-488. GSG1L immunoreactivity (green) appears in the outer molecular layer (OML) and in the granule layer (GL) of the hippocampal dentate gyrus. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- CRHERYPTRHQPH, corresponding to amino acid residues 283 - 295 of rat GSG1L (Accession D3ZK93).
Accession (Uniprot) Number D3ZK93
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
