Free shipping starts now, no minimum, no coupons required!

Guinea Pig Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody

5-Hydroxytryptamine receptor 2A, Serotonin receptor 2A, 5-HT2A

Cat #: ASR-033-GP
Alternative Name 5-Hydroxytryptamine receptor 2A, Serotonin receptor 2A, 5-HT2A
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea Pig
Reactivity: h, m, r
Immunogen
  • Peptide (C)SEASNWTIDAENR, corresponding to amino acid residues 40-52 of rat 5-HT2A (Accession P14842). Extracellular, N-terminus.
Accession (Uniprot) Number P14842
Gene ID 29595
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry
Homology Mouse - identical; human – 9 out of 13 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
5HT2A Receptor/HTR2A (extracellular) Blocking Peptide (#BLP-SR033)
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat dorsal root ganglion lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    Western blot analysis of rat dorsal root ganglion lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    1, 2. Guinea Pig Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033-GP), (1:200).
    3, 4. Guinea Pig Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody, preincubated with 5HT2A Receptor (HTR2A) (extracellular) Blocking Peptide (#BLP-SR033).
Immunohistochemistry
  • Expression of 5-HT2A in rat dorsal raphe nucleus.
    Expression of 5-HT2A in rat dorsal raphe nucleus.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033-GP), (1:400), followed by goat anti-guinea pig - Alexa Fluor-594. A. 5-HT2A immunoreactivity (red) appears in neuronal outlines (arrows).  B. Pre-incubation of the antibody with 5HT2A Receptor (HTR2A) (extracellular) Blocking Peptide (#BLP-SR033), suppressed staining. Cell nuclei are stained with DAPI (blue).
References
  1. Hutcheson, J.D. et al. (2011) Pharmacol. Ther. 132, 146.
  2. Fitzgerald, L.W. et al. (2000) Mol. Pharmacol. 57, 75.
  3. Lorke, D.E. et al. (2006) BMC Neurosci. 7, 36.
  4. Raote, I. et al. (2007) Serotonin receptors in neurobiology. FL: CRC Press. 105.
Scientific background

5-HT is involved in a diverse array of physiological and biological processes. In the brain, 5-HT affects sleep, mood, appetite, anxiety, aggression, perception, pain, and cognition1. Signaling of 5-HT is mediated by receptors that are located on the cell membrane of neurons and most other cells in the body. The 5-HT2 family consists of three G-protein coupled receptors (GPCRs): 5-HT2A, 5-HT2B, and 5-HT2C2. They are transmembrane proteins consisting of seven membrane-spanning α-helical segments with an extracellular N-terminus and an intracellular C-terminus. The binding of 5-HT to one of its receptors is thought to elicit a conformational change that activates associated heterotrimeric G proteins and recruits other downstream signaling/scaffolding molecules, such as GPCR kinases and β-arrestins.

5-HT2A receptors have been found in many regions of the human brain including the cortex, brainstem, olfactory bulb, limbic system and basal ganglia3.

The 5-HT2A receptor has been implicated in many mental disorders including schizophrenia, depression, obsessive compulsive disorder (OCD), attention deficit–hyperactivity disorder (ADHD), and eating disorders such as anorexia nervosa and autism spectrum disorders4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/08/2022

Guinea Pig Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033-GP) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize 5-HT2A from mouse, rat and human samples.

The antigen used to immunize guinea pigs is the same as Anti-5HT2A Receptor (HTR2A) (extracellular) (#ASR-033) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

For research purposes only, not for human use
Shipping and Ordering information