- Peptide (C)RDKYEPAAVSEHGD, corresponding to amino acid residues 7- 20 of rat ATP1A1 (Accession P06685). Intracellular, N-terminus.
Alpha 1 Na+/K+ ATPase Blocking Peptide (#BLP-NP001)
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Guinea Pig Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001-GP), (1:400).
3-4. Guinea Pig Anti-Alpha 1 Na+/K+ ATPase Antibody, preincubated with Alpha 1 Na+/K+ ATPase Blocking Peptide (#BLP-NP001).
- Expression of ATP1A1 in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Guinea Pig Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001-GP), (1:300), followed by goat anti-guinea pig - Alexa Fluor-594. A. ATP1A1 immunoreactivity (red) appears in the granule layer (G, arrows). B. Pre-incubation of the antibody with Alpha 1 Na+/K+ ATPase Blocking Peptide (#BLP-NP001), suppressed staining. Cell nuclei are stained with DAPI (blue). MOL = Molecular layer; G= Granule layer.
- Goldin, S.M. et al. (1977) J. Biol. Chem. 252, 5630.
- Jorgensen, P. L. and Andersen, J.P. (1988) J. Membr. Biol. 103, 95.
- Pietrini, G. et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 8414.
- Yoon, K. L. and Guidotti, G. (1994) J. Biol. Chem. 269, 28249.
- Ji, L. et al. (2009) Life Sci. 85, 788.
Na+/K+-ATPase, a membrane protein located on the plasma membrane of all animal cells, is involved in maintaining the Na+ and K+ gradients across the cell membrane1. It is ubiquitously expressed in neurons and helps maintain normal neuronal function2. The enzyme is composed of a catalytic α chain of 112 kDa. and α glycosylated β chain of 40–50 kDa. There are four isoforms of the α subunit (α1, α2, α3 and α4) and three isoforms of the β subunit (β1, β2 and β3) with different tissue distributions3.
The N-terminus is located on the cytoplasmic side of the membrane. There are four transmembrane segments in the first third of the polypeptide chain, a large cytoplasmic loop (LCL) comprising the second third of the chain and four to six transmembrane segments in the C-terminal third of the protein with the C-terminus also on the cytoplasmic side of the membrane4.
Na+/K+-ATPase abnormalities have been reported to be involved in several neurological diseases such as seizures, bipolar disorder, spongiform encephalopathy, and Alzheimer’s disease. Na+/K+-ATPase may also have implications in behavioral defects5.
Species reactivity key:
Guinea Pig Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001-GP) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ATP1A1 from mouse, rat and human samples.
The antigen used to immunize guinea pigs is the same as Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.