- Peptide (C)RATDYLQASAITRIPS, corresponding to amino acid residues 213-228 of mouse CaVγ2 (Accession O88602). Intracellular, C-terminus.
- Rat and mouse brain lysates (1:500-1:2500).
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Guinea pig Anti-Stargazin Antibody (#ACC-012-GP), (1:500).
3,4. Guinea pig Anti-Stargazin Antibody, preincubated with Stargazin Blocking Peptide (#BLP-CC012).
The γ subunit protein is an auxiliary subunit of the CaV (voltage dependent calcium) channel multimer complex. γ1 was first identified in skeletal muscle as a part of the proteins complex purified as the DHP receptor. Eight γ subunit isoforms were identified in mammalian genomes and they share some sequence as well as structure homology1. γ subunit contains four transmembrane helices, with intracellular N- and C- termini. Usually their association with the pore-forming α1 subunit results in reduced current amplitude.
CaVγ2 is also known as Stargazin, and interacts and regulates the activity and trafficking of AMPA glutamate receptors2.