Name: Guinea pig Anti-Stargazin Antibody
Brand: Alomone Labs
SKU: ACC-012-GP (formerly AGP-069)

Cat #: ACC-012-GP (formerly AGP-069)

Alternative Name Ca_Vγ2, Voltage-dependent calcium channel gamma-2 subunit, CACNG2, TARP gamma-2, Transmembrane AMPAR regulatory protein gamma-2, Waggler

Lyophilized Powder yes

Type: Polyclonal

Host: Guinea pig

Reactivity: m, r

May also work in: h*

Immunogen

Peptide (C)RATDYLQASAITRIPS, corresponding to amino acid residues 213-228 of mouse Ca_Vγ2 (Accession O88602). Intracellular, C-terminus.

Accession (Uniprot) Number O88602

Gene ID 12300

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse – 14/15 amino acid residues identical; human – 12/15 amino acid residues identical.

RRID AB_2756616.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Guinea pig total IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*

Western blot

Rat and mouse brain lysates (1:500-1:2500).
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
1,2. Guinea pig Anti-Stargazin Antibody (#ACC-012-GP), (1:500).
3,4. Guinea pig Anti-Stargazin Antibody, preincubated with Stargazin Blocking Peptide (#BLP-CC012).

Scientific background

The γ subunit protein is an auxiliary subunit of the Ca_V (voltage dependent calcium) channel multimer complex. γ1 was first identified in skeletal muscle as a part of the proteins complex purified as the DHP receptor. Eight γ subunit isoforms were identified in mammalian genomes and they share some sequence as well as structure homology¹.γ subunit contains four transmembrane helices, with intracellular N- and C- termini. Usually their association with the pore-forming α1 subunit results in reduced current amplitude.

Ca_Vγ2 is also known as Stargazin, and interacts and regulates the activity and trafficking of AMPA glutamate receptors².

Lyophilized Powder

Last update: 08/01/2023

For research purposes only, not for human use

Specifications

Citations

Applications

Scientific Background

Shipping and Ordering information

Control Product

Stargazin Blocking Peptide (#BLP-CC012) is the original antigen used for immunization during Anti-Stargazin Antibody (#ACC-012) generation. The blocking peptide binds and ‘blocks’ Anti-Stargazin primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Stargazin Blocking Peptide (#BLP-CC012)

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Order Summary

Certificate of Analysis

Guinea pig Anti-Stargazin Antibody

Application key:

Species reactivity key:

Specifications

Citations

Applications

Scientific Background

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