Guinea pig Anti-Stargazin Antibody

CaVγ2, Voltage-dependent calcium channel gamma-2 subunit, CACNG2, TARP gamma-2, Transmembrane AMPAR regulatory protein gamma-2, Waggler
    Cat #: AGP-069
    Alternative Name CaVγ2, Voltage-dependent calcium channel gamma-2 subunit, CACNG2, TARP gamma-2, Transmembrane AMPAR regulatory protein gamma-2, Waggler
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Guinea pig
    Reactivity: m, r
    May also work in: h*
      • Peptide (C)RATDYLQASAITRIPS, corresponding to amino acid residues 213-228 of mouse CaVγ2 (Accession O88602). Intracellular, C-terminus.
        Guinea pig Anti-Stargazin Antibody
    Accession (Uniprot) Number O88602
    Gene ID 12300
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – 14/15 amino acid residues identical; human – 12/15 amino acid residues identical.
    RRID AB_2756616.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Guinea pig total IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
      • Guinea pig Anti-Stargazin Antibody
        Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
        1,2. Guinea pig Anti-Stargazin Antibody (#AGP-069), (1:500).
        3,4. Guinea pig Anti-Stargazin Antibody, preincubated with the negative control antigen.
    References
    • 1. Arikkath, J. et al. (2003) Curr. Opin. Neurobiol. 13, 298.
    • 2. Chen, L. et al. (2000) Nature 408, 936.
      • The γ subunit protein is an auxiliary subunit of the CaV (voltage dependent calcium) channel multimer complex. γ1 was first identified in skeletal muscle as a part of the proteins complex purified as the DHP receptor. Eight γ subunit isoforms were identified in mammalian genomes and they share some sequence as well as structure homology1. γ subunit contains four transmembrane helices, with intracellular N- and C- termini. Usually their association with the pore-forming α1 subunit results in reduced current amplitude.

        CaVγ2 is also known as Stargazin, and interacts and regulates the activity and trafficking of AMPA glutamate receptors2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 22/09/2019

    Guinea pig Anti-Stargazin Antibody (#AGP-069) is a highly specific antibody directed against an epitope of the mouse CaVγ2 subunit. The antibody can be used in western blot analysis. It and has been designed to recognize CaVγ2 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-Stargazin Antibody (#ACC-012) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.

    For research purposes only, not for human use