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Guinea pig Anti-Connexin-43 Antibody

CX43, Gap junction α1 protein, GJA1, GJAL, Gap junction 43 kDa heart protein

Cat #: AGP-141
Alternative Name CX43, Gap junction α1 protein, GJA1, GJAL, Gap junction 43 kDa heart protein
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea pig
Reactivity: h, m, r
Immunogen
  • Peptide (C)HAQPFDFPDDNQNSK, corresponding to amino acids residues 331-345 of human Connexin-43 (Accession P17302). Intracellular, C-terminus.
Accession (Uniprot) Number P17302
Gene ID 2697
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - 14/15 amino acid residues identical; mouse - 13/15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 3), (1:1000) and mouse brain (lanes 2 and 4), (1:200) membranes:
    Western blot analysis of rat brain (lanes 1 and 3), (1:1000) and mouse brain (lanes 2 and 4), (1:200) membranes:
    1,2. Guinea pig Anti-Connexin-43 Antibody (#AGP-141). 
    3,4. Guinea pig Anti-Connexin-43 Antibody, preincubated with Connexin-43 Blocking Peptide (#BLP-CC201).
  • Western blot analysis of rat (lanes 5 and 7) and mouse (lanes 6 and 8) heart membranes:
    Western blot analysis of rat (lanes 5 and 7) and mouse (lanes 6 and 8) heart membranes:
    5,6. Guinea pig Anti-Connexin-43 Antibody (#AGP-141), (1:400).
    7,8. Guinea pig Anti-Connexin-43 Antibody, preincubated with Connexin-43 Blocking Peptide (#BLP-CC201).
  • Western blot analysis of human T-cell leukemia (Jurkat) (lanes 9 and 11) and human neuroblastoma (SH-SY5Y) (lanes 10 and 12) cell line lysates:
    Western blot analysis of human T-cell leukemia (Jurkat) (lanes 9 and 11) and human neuroblastoma (SH-SY5Y) (lanes 10 and 12) cell line lysates:
    9,10. Guinea pig Anti-Connexin-43 Antibody (#AGP-141), (1:400).
    11,12. Guinea pig Anti-Connexin-43 Antibody, preincubated with Connexin-43 Blocking Peptide (#BLP-CC201).
Immunohistochemistry
  • Expression of Connexin-43 in mouse brain
    Expression of Connexin-43 in mouse brain
    Immunohistochemical staining of frozen mouse cerebellum free floating sections using Guinea pig Anti-Connexin-43 Antibody (#AGP-141). CX43 immunoreactivity (red) appears in the Purkinje layer (vertical arrows) and in puncta along dendritic processes (horizontal arrows). Nuclei are stained using DAPI as the counterstain (blue).
References
  1. Goodenough, D.A. and Paul, D.L. (2009) Cold Spring Harb. Persp. Biol. 1, a002576.
  2. Nakagawa, S. et al. (2010) Curr. Opin. Struct. Biol. 20, 423.
  3. Chew, S.S. et al. (2010) Exp. Neurol. 225, 250.
  4. Trexler, E.B. et al. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 5836.
  5. Hu, X. et al. (2006) Biophys. J. 90, 140.
  6. Tang, Q. et al. (2009) J. Gen. Physiol. 133, 555.
Scientific background

Connexins (Cx) are integral membrane proteins consisting of four transmembrane domains, two extracellular loops, one intracellular loop and intracellular N- and C-termini. The 21 members belonging to this family form homomeric or heteromeric hexamers generally termed connexons or hemi-channels. In turn, these hemi-channels further assemble in a head-to-head manner, thus forming gap junction channels1,2. Connexins are ubiquitously expressed and their activity is regulated at the expression level and by post-translational modifications1. For example, Connexin-43 (Cx43) protein level is regulated by its turnover rate and by phosphorylation of various residues which ultimately determines its activity rate1.

Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells3. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2,4,5.

Due to their important roles, mutations in connexins are linked with a number of diseases such as neurodegenerative disorders, skin diseases, deafness, and developmental abnormalities2,5,6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Guinea pig Anti-Connexin-43 Antibody (#AGP-141), raised in guinea pigs, is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CX43 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Connexin-43 Antibody (#ACC-201) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

For research purposes only, not for human use
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