Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody

MR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480
  • New
Cat #: AGP-214
Alternative Name MR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Guinea pig
    Reactivity: h, m, r
    Immunogen
    • Peptide CKQGFLSSNGQNHFK, corresponding to amino acid residues 58-72 of human EMR1 (Accession Q14246). Extracellular, N-terminus.
    • Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody
    Accession (Uniprot) Number Q14246
    Gene ID 2015
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, Rat – 11/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Guinea pig total IgG
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*, lci*
    Western blot
    • Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody
      Western blot analysis of human promyelocytic leukemia (HL-60) (lanes 1 and 5), human Burkitt's lymphoma (Raji) B (lanes 2 and 6), mouse macrophage J774.1 (lanes 3 and 7) and rat basophilic leukemia (RBL) (lanes 4 and 8) cell lysates:
      1-4. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AGP-214), (1:200).
      5-8. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody, preincubated with the negative control antigen.
    • Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody
      Western blot analysis of rat and mouse fat tissue lysate:
      9, 10. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AGP-214), (1:200).
      11, 12. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody, preincubated with the negative control antigen.
    • Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody
      Western blot analysis of rat and mouse spleen lysate:
      13, 14. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AGP-214), (1:200).
      15, 16. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody, preincubated with the negative control antigen.
    References
    1. Legrand, F. et al. (2014) J. Allergy Clin. Immunol. 133, 1439.
    2. McKnight, A.J. and Gordon, S. (1998) J. Leukoc. Biol. 63, 271.
    3. Araç, D. et al. (2012) EMBO J. 31, 1364.
    Scientific background

    Human epidermal growth factor (EGF)–like module containing mucin-like hormone receptor 1 (EMR1) is a surface receptor with unknown function that belongs to the adhesion family of the G protein-coupled receptors (GPCR). This receptor is encoded by the ADGRE1 and like all GPCRs, contains seven transmembrane domains, an intracellular C-terminus and an extracellular N-terminus. The NH2 domain has six EGF-like domains.

    Adhesion GPCRs share a long N-terminal extracellular sequences comprising multiple domains, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties1,2.

    EMR1 protein is a homolog of F4/80, a murine protein that is widely being used as a marker of murine macrophage populations1.

    Human EMR1 is exclusively expressed on mature eosinophils in the blood and bone marrow and in nasal polyps. Thus, EMR1 is considered to be a potential therapeutic target for the treatment of eosinophilic disorders1,3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 17/02/2020

    Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AGP-214) raised in guinea pig, is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize ADGRE1 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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