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Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody

Mrgd, Mas-related G-protein coupled receptor member D, G-protein coupled receptor TGR7, β-Alanine receptor

Cat #: AGP-272
Alternative Name Mrgd, Mas-related G-protein coupled receptor member D, G-protein coupled receptor TGR7, β-Alanine receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea Pig
Reactivity: m, r
Immunogen
  • Peptide (C)KSHRLQESLGAVLGR, corresponding to amino acid residues 285-299 of rat MRGPRD (Accession Q7TN41). Intracellular, C-terminus.
Accession (Uniprot) Number Q7TN41
Gene ID 293648
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical. It will not recognize MRGPRD from human samples.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of rat dorsal root ganglion lysates:
    Western blot analysis of rat dorsal root ganglion lysates:
    1. Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody (#AGP-272), (1:500).
    2. Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody, preincubated with MRGPRD (GPCR TGR7) Blocking Peptide (BLP-MR061).
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) testis membranes:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) testis membranes:
    1-2. Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody (#AGP-272), (1:800).
    3-4. Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody, preincubated with MRGPRD (GPCR TGR7) Blocking Peptide (BLP-MR061).
References
  1. Uno, M. et al. (2012) J. Biomed. Biotechnol. 2012, 816159.
  2. Wang, H. et al. (2009) J. Neurosci. 29, 13202.
  3. Zylka, M.J. et al. (2003) Proc. Natl. Acad. Sci. USA. 100, 10043.
  4. Rau, K.K. et al. (2009) J. Neurosci. 29, 8612.
  5. Crozier, R.A. et al. (2007) J. Neurosci. 27, 4492.
Scientific background

Mas-related G-protein coupled receptor member D (MRGPRD) is expressed in the dorsal root ganglia, specifically in non-peptidergic, small-diameter, IB4+ C-fiber neurons that innervate the outer layers of the mammalian skin. They transmit polymodal nociceptive information from the skin epidermis to the substantia gelatinosa of the spinal cord.

MRGPRD receptor expressing neurons are essential for the full expression of mechanical nociception. Thus, MRGPRD represents an attractive therapeutic target for the development of agents for treating pain1,2.

The Mrg family in mice is comprised of six single-copy genes: MrgD, MrgE, MrgF/RTA, MrgG, MrgH/GPR90, and MAS1. In addition, there are three large subfamilies: MrgA, MrgB,and MrgC3.

MRGPRD receptor is activated by β-alanine and is able to inhibit KCNQ/M-type potassium channels and to increase excitability of sensory neurons4,5. MRGPRD has the ability to form heterodimers and is encoded by the TGR7 gene. This receptor is co-expressed with two nociceptors, including purinergic receptor (P2X3) and a vanilloid receptor (VR1)5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 20/07/2021

Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody (#AGP-272) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot applications. It has been designed to recognize MRGPRD (GPCR TGR7) from rat and mouse samples. The antibody will not recognize MRGPRD from human samples.

The antigen used to immunize guinea pigs is the same as Anti-MRGPRD (GPCR TGR7) Antibody (#AMR-061) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

For research purposes only, not for human use
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