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Guinea Pig Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody

nAChR α6, Cholinergic receptor nicotinic alpha 6, Neuronal acetylcholine receptor subunit alpha-6, ACRA6

Cat #: AGP-285
Alternative Name nAChR α6, Cholinergic receptor nicotinic alpha 6, Neuronal acetylcholine receptor subunit alpha-6, ACRA6
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea Pig
Reactivity: m, r
Immunogen
  • Peptide (C)EQLFHTLFAHYNR, corresponding to amino acid residues 35-47 of rat nAChRα6 (Accession P43143). Extracellular, N-terminus.
Accession (Uniprot) Number P43143
Gene ID 81721
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 12/13 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG
Specificity The antibody is not recommended for human samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of rat brain membrane lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    Western blot analysis of rat brain membrane lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    1-2. Guinea Pig Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody (#AGP-285), (1:200).
    3-4. Guinea Pig Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Blocking Peptide (BLP-NC006).
References
  1. Betz, H. (1990) Neuron 5, 383.
  2. Champtiaux, N. et al. (2003) J Neurosci23, 7820.
  3. le Novere, N. et al. (1999) Neuroreport 10, 2497.
  4. Quik, M. et al. (2007) Biochem. Pharmacol74, 1224.
  5. Hone, A.J. et al. (2012) FASEB J. 26, 917.
Scientific background

Neuronal nicotinic acetylcholine receptors (nAChRs) belong to the superfamily of ligand-gated ion channels and are widely expressed throughout the central and peripheral nervous systems. nAChRs play crucial roles in modulating a wide range of higher cognitive functions by mediating presynaptic, postsynaptic, and extrasynaptic signaling1.

Nicotinic acetylcholine receptors containing α6 subunits (nAChRα6) exhibit a unique expression pattern in the mammalian brain. nAChRs α6 are abundantly expressed in the midbrain dopaminergic (DAergic) system, including mesocorticolimbic and nigrostriatal pathways, and particularly present in presynaptic nerve terminals2. nAChRα6 expressed on DAergic neurons can be activated by endogenous acetylcholine (Ach) or exogenous nicotine and analogs, which suggests that the activation of nAChRα6 may play a vital role in central cholinergic circuits, including modulation of locomotor behaviour and drug addiction3. In addition, nAChRα6 is particularly susceptible to nigrostriatal damage, which may lead to Parkinson’s disease (PD)4. nAChRα6 are also expressed by sensory neurons; therefore, they may also play a role in disorders related to sensory perception5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/11/2021

Guinea Pig Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody (#AGP-285) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot. It has been designed to recognize nAChRα6 from mouse and rat samples. The antibody is not recommended for human samples.
The antigen used to immunize guinea pigs is the same as Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody (#ANC-006) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

For research purposes only, not for human use
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