Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody

P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3
    Cat #: AGP-098
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Guinea pig
    Reactivity: h, m, r
    Immunogen
    Peptide CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2RY12 (Accession Q9H244). 3rd extracellular loop.
    Accession (Uniprot) Number Q9H244
    Gene ID 64805
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – 12/13 amino acid residues identical; rat – 12/13 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, lci, wb
    May also work in: ifc, ih, ip
    Western blot
    Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    1,2. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#AGP-098), (1:400).
    3,4. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody, preincubated with the control peptide antigen.
    Western blot analysis of rat basophilic leukemia (RBL) cell line lysate (lanes 5 and 7) and human brain glioblastoma (U-87 MG) cell line lysate (lanes 6 and 8):
    5,6. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#AGP-098), (1:400).
    7,8. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody, preincubated with the control peptide antigen.
    Live cell imaging / Immunocytochemistry
    Expression of P2RY12 in human glioblastoma (U-87 MG) cells
    Immunocytochemical staining of intact living U-87 MG cells. A. Extracellular staining of cells using Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#AGP-098), (1:100) followed by goat anti-guinea pig-AlexaFluor-594 secondary antibody (red). DAPI was used as the counterstain (blue). B. Live view of the cells merged with P2Y12 staining.
    References
    1. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
    2. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
    3. Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci. 24, 52.
    4. Nicholas, R.A. (2001) Mol. Pharmacol. 60, 416.
    Scientific background

    The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1P2Y2P2Y4P2Y6P2Y11P2Y12P2Y13, and the UDP-glucose receptor, now renamed P2Y141-3

    The P2Y12 receptor is co-expressed with P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation.

    The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting4. The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 05/11/2018

    Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#AGP-098) is a highly specific antibody directed against an epitope of the human P2RY12. The antibody can be used in western blot and live imaging applications. It and has been designed to recognize P2RY12 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.

    For research purposes only, not for human use
    Citations
    Related Products
      • Products for control experiments
        1. Anti-P2Y12 Receptor Antibody (#APR-012).
          This antibody recognizes a different epitope and thus can also be used as a control for detecting P2Y12.
      • Antibodies for immuno-colocalization experiments
        1. Anti-P2Y12 Receptor-ATTO-594 Antibody (#APR-012-AR).
          A fluorescent labeled primary antibody. It can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit.