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Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody

P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3
Cat #: AGP-098
Alternative Name P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea pig
Reactivity: h, m, r
Immunogen
  • Peptide CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2RY12 (Accession Q9H244). 3rd extracellular loop.
Accession (Uniprot) Number Q9H244
Gene ID 64805
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – 12/13 amino acid residues identical; rat – 12/13 amino acid residues identical.
RRID AB_2756620.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, lci, wb
May also work in: ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    1,2. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#AGP-098), (1:400).
    3,4. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody, preincubated with P2Y12 Receptor (extracellular) Blocking Peptide (#BLP-PR020).
  • Western blot analysis of rat basophilic leukemia (RBL) cell line lysate (lanes 5 and 7) and human brain glioblastoma (U-87 MG) cell line lysate (lanes 6 and 8):
    Western blot analysis of rat basophilic leukemia (RBL) cell line lysate (lanes 5 and 7) and human brain glioblastoma (U-87 MG) cell line lysate (lanes 6 and 8):
    5,6. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#AGP-098), (1:400).
    7,8. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody, preincubated with P2Y12 Receptor (extracellular) Blocking Peptide (#BLP-PR020).
Live cell imaging / Immunocytochemistry
  • Expression of P2RY12 in human glioblastoma (U-87 MG) cells
    Expression of P2RY12 in human glioblastoma (U-87 MG) cells
    Cell surface detection of P2RY12 in intact living U-87 MG cells. A. Extracellular staining of cells using Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#AGP-098), (1:100) followed by goat anti-guinea pig-AlexaFluor-594 secondary antibody (red). DAPI was used as the counterstain (blue). B. Live view of the cells merged with P2Y12 staining.
References
  1. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
  2. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
  3. Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci. 24, 52.
  4. Nicholas, R.A. (2001) Mol. Pharmacol. 60, 416.
Scientific background

The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1P2Y2P2Y4P2Y6P2Y11P2Y12P2Y13, and the UDP-glucose receptor, now renamed P2Y141-3

The P2Y12 receptor is co-expressed with P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation.

The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting4. The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#AGP-098) is a highly specific antibody directed against an epitope of the human P2RY12. The antibody can be used in western blot and live imaging applications. It and has been designed to recognize P2RY12 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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