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Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody

Synaptotagmin I, SytI, p65, SVP65

Cat #: ANR-003-GP
Alternative Name Synaptotagmin I, SytI, p65, SVP65
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea Pig
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide MVSASHPEALAAPVTTVAT(C), corresponding to amino acid residues 1-19 of rat Synaptotagmin 1 (Accession P21107). Intravesicular, N- terminus.
Accession (Uniprot) Number P21707
Gene ID 25716
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – 18 out of 19 amino acid residues identical, human, bovine – 17 out of 19 amino acid residues identical, chicken – 15 out of 19 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat brain lysates:
    Western blot analysis of rat brain lysates:
    1. Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003-GP), (1:400).
    2. Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003-GP), preincubated with Synaptotagmin-1 (SYT1) Blocking Peptide (#BLP-NR003).
  • Western blot analysis of mouse brain lysates:
    Western blot analysis of mouse brain lysates:
    1. Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003-GP), (1:200).
    2. Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003-GP), preincubated with Synaptotagmin-1 (SYT1) Blocking Peptide (#BLP-NR003).
Immunohistochemistry
  • Expression of Synaptotagmin-1 in rat cerebellum.
    Expression of Synaptotagmin-1 in rat cerebellum.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003-GP), (1:1000), followed by goat anti-guinea pig - Alexa Fluor-594. A. Synaptotagmin-1 immunoreactivity (red) appears in the granule layer (vertical arrows) and in the molecular layer (horizontal arrows).  B. Pre-incubation of the antibody with Synaptotagmin-1 (SYT1) Blocking Peptide (#BLP-NR003), suppressed staining. Cell nuclei are stained with DAPI (blue). M = molecular layer, P = purkinje layer, G = granule layer.
  • Expression of Synaptotagmin-1 in rat spinal cord.
    Expression of Synaptotagmin-1 in rat spinal cord.
    Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003-GP), (1:1000), followed by goat anti-guinea pig - Alexa Fluor-594. A. Staining in the spinal dorsal horn region, showed Synaptotagmin-1 immunoreactivity (red) in axonal outlines in the superficial layer of the dorsal horn (arrows).  B. Pre-incubation of the antibody with Synaptotagmin-1 (SYT1) Blocking Peptide (#BLP-NR003), suppressed staining. Cell nuclei are stained with DAPI (blue).
References
  1. Südhof, T.C. (2002) J. Biol. Chem. 277, 7629.
  2. Malsam, J. et al. (2008) Cell. Mol. Life Sci. 65, 2814.
  3. Meir, A. et al. (1999) Physiol. Rev. 79, 1019.
Scientific background

Synaptotagmin 1 is a member of the Ca2+-binding synaptotagmin family of proteins that includes 16 members in vertebrates and is involved in regulated exocytosis and trafficking1,2.

Synaptotagmins are type I membrane proteins located in intracellular vesicles and consist of a short N-terminal domain located in the vesicle lumen, a transmembrane domain and a cytosolic domain containing two Ca2+ binding domains1,2.

Synaptotagmins are usually localized to distinct secretory vesicles and control their Ca2+-dependent fusion. Synaptotagmin I is predominantly expressed in neuronal cells and is an abundant constituent of synaptic vesicles1,2.

Synaptotagmin 1 has a relatively low Ca2+ affinity and is able to bind Ca2+ mostly in the vicinity of Ca2+ channels pores. Indeed, Synaptotagmin 1 has been demonstrated to directly interact with selected Ca2+ channels. Synaptotagmin 1 also binds to the t-SNARE protein Syntaxin 1 in a Ca2+-dependent manner thus forming the prototypical Ca2+-dependent fast synaptic vesicle exocytosis protein complex that also includes SNAP-25 and VAMP1,2,3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 13/06/2022

Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003-GP) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Synaptotagmin-1 from mouse, rat and human samples.

The antigen used to immunize guinea pigs is the same as Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

For research purposes only, not for human use
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