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2. Guinea pig Anti-TRPC6 Antibody, preincubated with the control peptide antigen.
- Ramsey, S.I. et al. (2006) Annu. Rev. Physiol. 68, 619.
- Pedersen, S.F. et al. (2005) Cell. Calcium. 38, 233.
- Montell, C. (2005) Sci. STKE 2005, re3.
- Hilgemann, D.W. et al. (2001) Sci. STKE 2001, re19.
- Abramowitz, J. et al. (2009) FASEB J. 23, 297.
- Winn, M.P. et al. (2005) Science, 308, 1801.
- Reiser, J. et al. (2005) Nat. Genet. 37, 739.
Transient receptor potential (TRP) channels are relatively non-selective ion channels enabling the exchange of cations down their electrochemical gradient. This exchange enables the intracellular rise in Na+ and Ca2+ concentration and ultimately in the cell membrane depolarization, important for action potential propagation and muscle contraction1. They are activated by an extremely broad range of stimuli namely, temperature, voltage, pH, endocrine factors as well as signaling molecules2.
The TRP channel family is composed of 28 members divided in 7 subgroups: TRPV, TRPC, TRPM, TRPA, TRPN, TRPP and TRPML. All members of the TRP family form tetramers and could heterhomultimerize. They have 6 transmembrane (TM) domains, and a pore domain between the fifth (S5) and sixth (S6) transmembrane domains. In general, TRP channels enable the passage of either Na+ or Ca2+ ions with little or no preference. However, some channels do exhibit some selectivity. Also, TRP channels do not display the positive charges in the S4 voltage-sensing domain like most voltage sensitive channels, although they do display voltage dependency3. In addition, TRP channels have in the C-terminal intracellular region to the S6 domain a TRP domain comprising 25 amino acids that is more or less conserved among most TRP channels. Within the TRP domain, there is a TRP box composed of six amino acids, and TRP box 2 – a proline rich domain1,3. The TRP domain seems to be responsible for the binding of PIP2, a phospholipid important for the regulation of channel activity4.
The TRPC subfamily is further divided into the following: TRPC1/4/5, TRPC3/6/7 and TRPC21. Activation of phospholipase C (PLC) ultimately leads to the formation of diacylglycerol (DAG) and inositol 1,4,5-triphosphate (IP3) via hydrolysis of PIP2. The increase in concentration of these intracellular second messengers leads to the activation of non-selective Ca2+ channels and an IP3-induced release of Ca2+ from intracellular stores5. The intracellular Ca2+ store depletion in turn activates Ca2+ specific channels to allow replenish intracellular Ca2+ levels. TRPCs are thought to be activated upon intracellular store Ca2+ depletion, and may function in concert with the recently identified Orai channel5. The TRPC3/6/7 class produces similar currents upon activation.
TRPC6 can form heterotetramers with TRPC3 and TRPC7. It is primarily expressed in brain, lung and muscle. High levels of expression of the channel were also found in human platelets. Recently it was reported that TRPC6 is also expressed in the kidney where a mutated channel has been implicated in kidney failure disease6,7.
Species reactivity key:
Guinea pig Anti-TRPC6 Antibody (#AGP-002), raised in guinea pigs, is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TRPC6 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-TRPC6 Antibody (#ACC-017) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.
- Anti-TRPC6 Antibody (#ACC-017)
- Anti-TRPC6 (extracellular) Antibody (#ACC-120)
- Anti-TRPC1 Antibody (#ACC-010)
- Anti-TRPC2 Antibody (#ACC-027)
- Anti-TRPC3 Antibody (#ACC-016)
- Anti-TRPC3-ATTO-594 Antibody (#ACC-016-AR)
- Anti-TRPC4 Antibody (#ACC-018)
- Anti-TRPC5 Antibody (#ACC-020)
- Anti-TRPC7 (extracellular) Antibody (#ACC-066)