Guinea pig Anti-TRPV4 Antibody

OTRPC4, VRL-2, VR-OAC, TRP12
  • New
Cat #: AGP-147
Alternative Name OTRPC4, VRL-2, VR-OAC, TRP12
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Guinea pig
    Reactivity: r
    May also work in: h*, m*
    Immunogen
    • Peptide CDGHQQGYAPKWRAEDAPL, corresponding to amino acid residues 853-871 of rat TRPV4 (Accession Q9ERZ8). Intracellular, C-terminus.
    • Guinea pig Anti-TRPV4 Antibody
    Accession (Uniprot) Number Q9ERZ8
    Gene ID 66026
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 17/19 amino acid residues identical; human - 16/19 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Guinea pig total IgG
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
    Western blot
    • Guinea pig Anti-TRPV4 Antibody
      Western blot analysis of rat brain membranes:
      1. Guinea pig Anti-TRPV4 Antibody (#AGP-147), (1:1500).
      2. Guinea pig Anti-TRPV4 Antibody, preincubated with the negative control antigen.
    • Guinea pig Anti-TRPV4 Antibody
      Western blot analysis of rat DRG lysates:
      3. Guinea pig Anti-TRPV4 Antibody (#AGP-147), (1:200).
      4. Guinea pig Anti-TRPV4 Antibody, preincubated with the negative control antigen.
    References
    1. Montell, Cet al. (2002) Mol. Cell9, 229.
    2. Clapham, D.E. (2003) Nature 426, 517.
    3. Moran, M.M. et al. (2004) Curr.Opin.Neurobiol. 14, 362.
    4. Clapham, D.Eet al. (2003) Pharmacol. Rev55, 591.
    5. Gunthorpe, M.J. et al. (2002) Trends. Pharmacol. Sci. 23, 183.
    6. Peng, J.Bet al. (2003) J. Physiol551.3, 729.
    Scientific background

    TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells1. According to IUPHAR, the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPCTRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPATRPML, and TRPP)1-4. The TRPV subfamily consists of six members, TRPV1-65.

    TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 17/02/2020

    Guinea pig Anti-TRPV4 Antibody (#AGP-147), raised in guinea pig, is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize TRPV4 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-TRPV4 Antibody (#ACC-034) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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