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Certificate of Analysis

HCN1 Blocking Peptide

Overview

Cat #: BLP-PC056
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: bl

Application key:

BL- Blocking
For research purposes only, not for human use

Properties

Properties

Sequence
  • (C)KPNSASNSRDDGNSVYPSK, corresponding to amino acid residues 6-24 of rat HCN1 (Accession Q9JKB0).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.

Applications

Applications

Blocking
  • Western blot analysis of rat brain membrane:
    Western blot analysis of rat brain membrane:
    1. Anti-HCN1 Antibody (#APC-056), (1:200).
    2. Anti-HCN1 Antibody, preincubated with HCN1 Blocking Peptide (#BLP-PC056).
  • Mouse cerebellum frozen section.
  • Western blot analysis of rat brain lysate:
    Western blot analysis of rat brain lysate:
    1. Guinea pig Anti-HCN1 Antibody (#AGP-203), (1:500).
    2. Guinea pig Anti-HCN1 Antibody, preincubated with HCN1 Blocking Peptide (#BLP-PC056).
  • Rat cerebellum sections.
Last update: 11/04/2021
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