Overview
Type: GST fusion protein
Form: Lyophilized powder
HCN4 Blocking Peptide (#BLP-PC052) is the original antigen used for immunization during Anti-HCN4 Antibody (#APC-052) generation. The blocking peptide binds and ‘blocks’ Anti-HCN4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-HCN4 Antibody (#APC-052), (1:200).
2. Anti-HCN4 Antibody, preincubated with HCN4 Blocking Peptide (#BLP-PC052).
Expression of HCN4 in mouse thalamusImmunohistochemical staining of mouse thalamus using Anti-HCN4 Antibody (#APC-052). A. HCN4 (red) appears in the neuropil of the lateral nucleus (LN). B. Staining of reticular nucleus (RN) with mouse anti-Parvalbumin. C. Confocal merge of HCN4 and Parvalbumin images demonstrates separate localization of these proteins in the thalamus.
Western blot analysis of rat brain lysate:1. Guinea pig Anti-HCN4 Antibody (#APC-052-GP), (1:200).
2. Guinea pig Anti-HCN4 Antibody, preincubated with HCN4 Blocking Peptide (#BLP-PC052).
Expression of HCN4 in mouse thalamusImmunohistochemical staining of mouse thalamus using Guinea pig Anti HCN4 Antibody (#APC-052-GP). A. HCN4 staining (green) appears in the neuropil of the ventral posterior thalamic nucleus (VPL). B. Nuclear staining using DAPI as the counter stain (blue). C. Merged images of A and B.
Multiplex staining of HCN4 and HCN2 in mouse thalamusImmunohistochemical staining of mouse thalamus frozen section using Guinea pig Anti-HCN4 Antibody (#APC-052-GP) and rabbit Anti-HCN2 Antibody (#APC-030). A. Staining of HCN4 (green) appears in the ventral posterior thalamic nucleus (VPL). B. In the same section as in A, staining of HCN2 (red) appears in the ventral posterior thalamic nucleus (VPL) and also in the reticular thalamic nucleus (RT). The area between these thalamic nuclei (star) is white matter and neither protein is expressed in that region. C. Merged images of A and B.
Properties
Sequence
- GST fusion protein with the sequence HGHLHDSAEERRLIAEGDASPG EDRTPPGLAAEPERP, corresponding to amino acid residues 119-155 of human HCN4 (Accession Q9Y3Q4).
Accession (Uniprot) Number Q9Y3Q4
Peptide Confirmation Confirmed by DNA sequence and SDSPAGE.
Purity >95% (SDS-PAGE)
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 μl PBS.
Concentration After Reconstitution 1.2 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 3 μg fusion protein per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
