Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Histamine H 1 Receptor/HRH1 (extracellular) Blocking Peptide (#BLP-HR006) is the original antigen used for immunization during Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody (#AHR-006) generation. The blocking peptide binds and ‘blocks’ Anti-Histamine H 1 Receptor/HRH1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 5), rat brain membranes (lanes 2 and 6), rat lung membranes (lanes 3 and 7) and mouse lung membranes (lanes 4 and 8):1-4. Anti- Histamine H1 Receptor/HRH1 (extracellular) Antibody (#AHR-006), (1:200).
5-8. Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody, preincubated with Histamine H1 Receptor/HRH1 (extracellular) Blocking Peptide (#BLP-HR006).
Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 4), human U-87 MG glioblastoma cell line lysate (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6):1-3. Anti- Histamine H1 Receptor/HRH1 Antibody (extracellular) (#AHR-006), (1:200).
4-6. Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody, preincubated with Histamine H1 Receptor/HRH1 (extracellular) Blocking Peptide (#BLP-HR006).
Expression of Histamine H1 Receptor in mouse substantia nigraImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Histamine H1 Receptor/HRH1 Antibody (extracellular) (#AHR-006), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Histamine H1 Receptor immunoreactivity (green) appears in neuronal profiles (vertical arrows). B. Pre-incubation of the antibody with Histamine H1 Receptor (HRH1) (extracellular) Blocking Peptide (#BLP-HR006), suppresses staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)REDK(S)ETDFYNVTWFK, corresponding to amino acid residues 176 - 191 of mouse H1 Histamine Receptor (Accession P70174).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
